Skip to main content

Main menu

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • ERS Guidelines
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • Open access
    • Peer reviewer login
  • Alerts
  • Subscriptions
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

User menu

  • Log in
  • Subscribe
  • Contact Us
  • My Cart

Search

  • Advanced search
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

Login

European Respiratory Society

Advanced Search

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • ERS Guidelines
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • Open access
    • Peer reviewer login
  • Alerts
  • Subscriptions

Circulating Plasma Exosomes in Obstructive Sleep Apnea and Reverse-Dipping Blood Pressure

Abdelnaby Khalyfa, David Gozal, Wen-Ching Chan, Jorge Andrade, Bharati Prasad
European Respiratory Journal 2019; DOI: 10.1183/13993003.01072-2019
Abdelnaby Khalyfa
1Department of Child Health and the Child Health Research Institute, University of Missouri School of Medicine, Columbia, MO, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Abdelnaby Khalyfa
David Gozal
1Department of Child Health and the Child Health Research Institute, University of Missouri School of Medicine, Columbia, MO, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for David Gozal
Wen-Ching Chan
2Center for Research Informatics, The University of Chicago, Chicago, Illinois, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jorge Andrade
2Center for Research Informatics, The University of Chicago, Chicago, Illinois, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bharati Prasad
3Department of Pulmonary, Critical Care, Sleep and Allergy, University of Illinois at Chicago, Chicago, Illinois, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: bpradsad@uic.edu
  • Article
  • Info & Metrics
  • PDF
Loading

Abstract

Background Obstructive Sleep Apnea (OSA) increases the risk of abnormal non-dipping 24-hour blood pressure profile, an independent risk factor for cardiovascular disease (CVD). We examined differential exosomal miRNA expression in untreated OSA patients with normal dipping blood pressure (NDBP) and reverse dipping blood pressure (RDBP; an extreme form of non-dipping) to understand the mechanisms underlying non-dipping blood pressure in OSA.

Methods 46 patients (RDBP, n=15 versus NDBP, n=31) matched for OSA severity (respiratory event index, 32.6±22.5 h−1 versus 32.2±18.1 h−1, p=0.9), age (54.8±12.9 versus 49±9.9 years, p=0.09) and body mass index (36.2±6.6 kg·m−2 versus 34.4±6.8 kg·m−2, p=0.4) were included. Plasma exosomes were characterised by flow cytometry, and functional in vitro reporter assays were conducted on cultured endothelial cells. Exosome miRNA cargo was profiled with microarrays followed by bioinformatics analyses.

Results Exosomes from RDBP patients increased the permeability of endothelial cell tight-junctions and adhesion molecule expression. Principal component analyses of miRNA array data showed strict separation and identification of the two groups. A restricted and validated signature of exosomal miRNAs were identified in RDBP versus NDBP group. Their predicted target genes involved PI3K-Akt (p=0.004), Ras (p=3.42E-05), Wnt (p=0.003), and HIF-1 signalling (p=0.04), inflammatory mediator regulation of TRP channels (p=0.01), and several cancer-related pathways.

Conclusions Patients with RDBP have altered miRNA cargo in circulating exosomes that invoke in vitro endothelial dysfunction. A selected number of circulating exosomal miRNAs play an important role in abnormal circadian regulation of blood pressure and may provide prognostic biomarkers of CVD risk in OSA.

Footnotes

This manuscript has recently been accepted for publication in the European Respiratory Journal. It is published here in its accepted form prior to copyediting and typesetting by our production team. After these production processes are complete and the authors have approved the resulting proofs, the article will move to the latest issue of the ERJ online. Please open or download the PDF to view this article.

Conflict of interest: Dr. Khalyfa has nothing to disclose.

Conflict of interest: Dr. Gozal has nothing to disclose.

Conflict of interest: Dr. Chan has nothing to disclose.

Conflict of interest: Dr. Andrade has nothing to disclose.

Conflict of interest: Dr. Prasad has nothing to disclose.

This is a PDF-only article. Please click on the PDF link above to read it.

  • Received May 30, 2019.
  • Accepted October 12, 2019.
  • Copyright ©ERS 2019
PreviousNext
Back to top
View this article with LENS
Vol 62 Issue 3 Table of Contents
European Respiratory Journal: 62 (3)
  • Table of Contents
  • Index by author
Email

Thank you for your interest in spreading the word on European Respiratory Society .

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Circulating Plasma Exosomes in Obstructive Sleep Apnea and Reverse-Dipping Blood Pressure
(Your Name) has sent you a message from European Respiratory Society
(Your Name) thought you would like to see the European Respiratory Society web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Print
Citation Tools
Circulating Plasma Exosomes in Obstructive Sleep Apnea and Reverse-Dipping Blood Pressure
Abdelnaby Khalyfa, David Gozal, Wen-Ching Chan, Jorge Andrade, Bharati Prasad
European Respiratory Journal Jan 2019, 1901072; DOI: 10.1183/13993003.01072-2019

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Circulating Plasma Exosomes in Obstructive Sleep Apnea and Reverse-Dipping Blood Pressure
Abdelnaby Khalyfa, David Gozal, Wen-Ching Chan, Jorge Andrade, Bharati Prasad
European Respiratory Journal Jan 2019, 1901072; DOI: 10.1183/13993003.01072-2019
del.icio.us logo Digg logo Reddit logo Technorati logo Twitter logo CiteULike logo Connotea logo Facebook logo Google logo Mendeley logo
Full Text (PDF)

Jump To

  • Article
    • Abstract
  • Info & Metrics
  • PDF

Subjects

  • Sleep medicine
  • Tweet Widget
  • Facebook Like
  • Google Plus One

More in this TOC Section

  • Lung volumes and survival in chronic lung allograft dysfunction
  • Prior CV disease and mortality COPD pneumonia patients
  • Factors associated with FEV1 decline in CF
Show more Original article

Related Articles

Navigate

  • Home
  • Current issue
  • Archive

About the ERJ

  • Journal information
  • Editorial board
  • Press
  • Permissions and reprints
  • Advertising

The European Respiratory Society

  • Society home
  • myERS
  • Privacy policy
  • Accessibility

ERS publications

  • European Respiratory Journal
  • ERJ Open Research
  • European Respiratory Review
  • Breathe
  • ERS books online
  • ERS Bookshop

Help

  • Feedback

For authors

  • Instructions for authors
  • Publication ethics and malpractice
  • Submit a manuscript

For readers

  • Alerts
  • Subjects
  • Podcasts
  • RSS

Subscriptions

  • Accessing the ERS publications

Contact us

European Respiratory Society
442 Glossop Road
Sheffield S10 2PX
United Kingdom
Tel: +44 114 2672860
Email: journals@ersnet.org

ISSN

Print ISSN:  0903-1936
Online ISSN: 1399-3003

Copyright © 2023 by the European Respiratory Society