Skip to main content

Main menu

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • Open access
    • COVID-19 submission information
    • Peer reviewer login
  • Alerts
  • Podcasts
  • Subscriptions
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

User menu

  • Log in
  • Subscribe
  • Contact Us
  • My Cart

Search

  • Advanced search
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

Login

European Respiratory Society

Advanced Search

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • Open access
    • COVID-19 submission information
    • Peer reviewer login
  • Alerts
  • Podcasts
  • Subscriptions

Acute wheeze-specific gene module shows correlation with vitamin D and asthma medication

Shintaro Katayama, Katarina Stenberg Hammar, Kaarel Krjutškov, Elisabet Einarsdottir, Gunilla Hedlin, Juha Kere, Cilla Söderhäll
European Respiratory Journal 2019; DOI: 10.1183/13993003.01330-2019
Shintaro Katayama
1Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Katarina Stenberg Hammar
2Astrid Lindgren Children's Hospital, Karolinska University Hospital, Stockholm, Sweden
3Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kaarel Krjutškov
1Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden
4Competence Centre on Health Technologies, Tartu, Estonia
5Folkhälsan Institute of Genetics, and Stem Cells and Metabolism Research Program, University of Helsinki, Helsinki, Finland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Elisabet Einarsdottir
1Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden
5Folkhälsan Institute of Genetics, and Stem Cells and Metabolism Research Program, University of Helsinki, Helsinki, Finland
6Current affiliation: SciLifeLab, Department of Gene Technology, KTH-Royal Institute of Technology, Solna, Sweden
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gunilla Hedlin
2Astrid Lindgren Children's Hospital, Karolinska University Hospital, Stockholm, Sweden
3Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Juha Kere
1Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden
5Folkhälsan Institute of Genetics, and Stem Cells and Metabolism Research Program, University of Helsinki, Helsinki, Finland
7School of Basic & Medical Biosciences, King's College London, Guy's Hospital, London, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Cilla Söderhäll
1Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden
3Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: cilla.soderhall@ki.se
  • Article
  • Info & Metrics
  • PDF
Loading

Abstract

Background Airway obstruction and wheezing in preschool children with recurrent viral infections are a major clinical problem, and recognised as a risk factor for the development of chronic asthma. We aimed at analyaing whether gene expression profiling provides evidence for pathways that delineate distinct groups of children with wheeze, and in combination with clinical information could contribute to diagnosis and prognosis of disease development.

Methods We analyaed leukocyte transcriptomes from preschool children (6 months–3 y) at acute wheeze (n=107), and at a revisit 2–3 months later, comparing them to age-matched healthy controls (n=66). RNA-sequencing applying GlobinLock was used. The cases were clinically followed until age 7 years. Differential expression tests, weighted correlation network analysis (WGCNA) and logistic regression were applied and correlations to 76 clinical traits evaluated.

Findings Significant enrichment of genes involved in the innate immune responses were observed in children with wheeze. We identified a unique acute wheeze-specific gene-module, that was associated with Vitamin D levels (p<0.005) in infancy, and asthma medication and FEV1%/FVC several years later, at age 7 (p<0.005). A model that predicts LTRA-medication at 7 years of age with high accuracy was developed (AUC=0.815, 95% CI: 0.668–0.962).

Interpretation Gene expression profiles in blood from preschool wheezers predict asthma symptoms at school-age, and therefore serve as biomarkers. The acute wheeze-specific gene module suggests that molecular phenotyping in combination with clinical information already at an early episode of wheeze may help to distinguish children that will outgrow their wheeze from those that will develop chronic asthma.

Footnotes

This manuscript has recently been accepted for publication in the European Respiratory Journal. It is published here in its accepted form prior to copyediting and typesetting by our production team. After these production processes are complete and the authors have approved the resulting proofs, the article will move to the latest issue of the ERJ online. Please open or download the PDF to view this article.

Conflict of interest: Elisabet Einarsdottir

Conflict of interest: Dr. Hedlin has nothing to disclose.

Conflict of interest: Dr. Kere reports other from Blueprint Genetics, Inc., Helsinki, Finland., outside the submitted work; In addition, Dr. Kere has a patent GlobinLock method. pending.

Conflict of interest: Dr. Söderhäll has nothing to disclose.

Conflict of interest: Dr. Stenberg Hammar has nothing to disclose.

Conflict of interest: I state I have a patent “GlobinLock - blocking oligonucleotides” pending.

Conflict of interest: Dr. Katayama has nothing to disclose.

This is a PDF-only article. Please click on the PDF link above to read it.

  • Received January 24, 2019.
  • Accepted October 7, 2019.
  • Copyright ©ERS 2019
PreviousNext
Back to top
View this article with LENS
Vol 59 Issue 5 Table of Contents
European Respiratory Journal: 59 (5)
  • Table of Contents
  • Index by author
Email

Thank you for your interest in spreading the word on European Respiratory Society .

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Acute wheeze-specific gene module shows correlation with vitamin D and asthma medication
(Your Name) has sent you a message from European Respiratory Society
(Your Name) thought you would like to see the European Respiratory Society web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Print
Citation Tools
Acute wheeze-specific gene module shows correlation with vitamin D and asthma medication
Shintaro Katayama, Katarina Stenberg Hammar, Kaarel Krjutškov, Elisabet Einarsdottir, Gunilla Hedlin, Juha Kere, Cilla Söderhäll
European Respiratory Journal Jan 2019, 1901330; DOI: 10.1183/13993003.01330-2019

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Acute wheeze-specific gene module shows correlation with vitamin D and asthma medication
Shintaro Katayama, Katarina Stenberg Hammar, Kaarel Krjutškov, Elisabet Einarsdottir, Gunilla Hedlin, Juha Kere, Cilla Söderhäll
European Respiratory Journal Jan 2019, 1901330; DOI: 10.1183/13993003.01330-2019
del.icio.us logo Digg logo Reddit logo Technorati logo Twitter logo CiteULike logo Connotea logo Facebook logo Google logo Mendeley logo
Full Text (PDF)

Jump To

  • Article
    • Abstract
  • Info & Metrics
  • PDF

Subjects

  • Paediatric pulmonology
  • Asthma and allergy
  • Tweet Widget
  • Facebook Like
  • Google Plus One

More in this TOC Section

  • Lung volumes and survival in chronic lung allograft dysfunction
  • Automated quantification of radiological patterns predicts IPF survival
  • Aspergillus fumigatus in COPD
Show more Original article

Related Articles

Navigate

  • Home
  • Current issue
  • Archive

About the ERJ

  • Journal information
  • Editorial board
  • Reviewers
  • Press
  • Permissions and reprints
  • Advertising

The European Respiratory Society

  • Society home
  • myERS
  • Privacy policy
  • Accessibility

ERS publications

  • European Respiratory Journal
  • ERJ Open Research
  • European Respiratory Review
  • Breathe
  • ERS books online
  • ERS Bookshop

Help

  • Feedback

For authors

  • Instructions for authors
  • Publication ethics and malpractice
  • Submit a manuscript

For readers

  • Alerts
  • Subjects
  • Podcasts
  • RSS

Subscriptions

  • Accessing the ERS publications

Contact us

European Respiratory Society
442 Glossop Road
Sheffield S10 2PX
United Kingdom
Tel: +44 114 2672860
Email: journals@ersnet.org

ISSN

Print ISSN:  0903-1936
Online ISSN: 1399-3003

Copyright © 2022 by the European Respiratory Society