Abstract
Introduction: Adenocarcinoma(ADENO) and squamous cell carcinoma(SCC) are complex diseases that involves molecular alterations and immune system, which is a key regulator for immunotherapy. Dendritic cells(DC) play a critical role in the tumor microenvironment in pro-active anti-tumor immune stimulation or in a “protective” system.
Aims and Objectives: To determine the dendritic cell profile in adenocarcinoma and squamous cell carcinoma.
Methods: A retrospective study was carried out with 46 NSCLC lobectomy samples. A detailed evaluation of H&E slides and immunohistochemical panel for CD1a, Langerin and Mannose were performed with morphometrical analysis.
Results: Survival analysis revealed that CD1a+DC has worst survival in ADENO with necrosis area less than 50%(P<0.05). Therefore, the immune stimulation of CD1a+DC and Langerin+DC in ADENO appeared as ineffective in tumor and front area. However, these immunophenotypes in SCC did not present significant participation, not affecting survival. Furthermore, both ADENO and SCC revealed Mannose+DC as an important role in the front area.
Conclusions: The expression of dendritic cells in the tumor microenvironment differs between ADENO and CEC. In ADENO, some molecular mechanisms may be preventing the proper functioning of DC with immunophenotype CD1a+ and Langerin+, while in SCC, other molecular pathways may be blocking their expression.(FAPESP Nº22/02821-0, 21/09024-6, 21/10981-5, 19/01517-3).
Footnotes
Cite this article as: European Respiratory Journal 2023; 62: Suppl. 67, OA778.
This abstract was presented at the 2023 ERS International Congress, in session “Inflammatory endotyping: the macrophage across disease areas”.
This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).
- Copyright ©the authors 2023
