Abstract
Introduction: Treatment for lung cancer patients requires a tissue diagnosis. To date, the absence of on-site feedback on the quality of histology samples regularly results in non-diagnostic and repeat procedures. Higher harmonic generation microscopy (HHGM) provides rapid histological images of unprocessed tissue.
Aim: Assess the feasibility of HHGM for instant bronchoscopic and pleural biopsy pathological quality assessment.
Methods: Fresh biopsies of patients undergoing diagnostic bronchoscopy or thoracoscopy were imaged with a mobile HHG microscope (Flash Pathology B.V.) and subsequently processed for routine diagnostics. Dedicated lung pathologists assessed independently the HHGM image quality for pathological assessment.
Results: In 47 patients, final diagnosis: malignant (16), non-malignant (29), non-representative (2), 107 biopsies were evaluated. HHGM provided total biopsy scans within 1-2 minutes and generated a high, cellular resolution image within an additional 3 minutes (Figure1). The HHGM image quality was adequate for 89% and 94% of the biopsies according to 2 independent pathologists (inter-observer agreement of 87%). Next, at least 2 pathologists will compare both HHGM and standard histology images to set HHGM malignancy criteria for HHGM biopsy quality/diagnosis.
Conclusion: HHGM for on-site rapid assessment of bronchoscopic and pleural biopsies is feasible. Its clinical value requires further research.
Footnotes
Cite this article as Eur Respir J 2022; 60: Suppl. 66, 3791.
This article was presented at the 2022 ERS International Congress, in session “-”.
This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).
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