Skip to main content

Main menu

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • Open access
    • COVID-19 submission information
    • Peer reviewer login
  • Alerts
  • Podcasts
  • Subscriptions
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

User menu

  • Log in
  • Subscribe
  • Contact Us
  • My Cart

Search

  • Advanced search
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

Login

European Respiratory Society

Advanced Search

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • Open access
    • COVID-19 submission information
    • Peer reviewer login
  • Alerts
  • Podcasts
  • Subscriptions

Murine precision cut lung slices as a novel 3D-model for studying endocytic pathways in the lung

Vitalii Kryvenko, Andrés Alberro Brage, Werner Seeger, Susanne Herold, Christos Samakovlis, István Vadász
European Respiratory Journal 2021 58: PA3698; DOI: 10.1183/13993003.congress-2021.PA3698
Vitalii Kryvenko
1Department of Internal Medicine, Justus Liebig University, Universities of Giessen and Marburg Lung Center, Member of the German Center for Lung Research, Giessen, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: Vitalii.Kryvenko@innere.med.uni-giessen.de
Andrés Alberro Brage
1Department of Internal Medicine, Justus Liebig University, Universities of Giessen and Marburg Lung Center, Member of the German Center for Lung Research, Giessen, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Werner Seeger
1Department of Internal Medicine, Justus Liebig University, Universities of Giessen and Marburg Lung Center, Member of the German Center for Lung Research, Giessen, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Susanne Herold
1Department of Internal Medicine, Justus Liebig University, Universities of Giessen and Marburg Lung Center, Member of the German Center for Lung Research, Giessen, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Christos Samakovlis
1Department of Internal Medicine, Justus Liebig University, Universities of Giessen and Marburg Lung Center, Member of the German Center for Lung Research, Giessen, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
István Vadász
1Department of Internal Medicine, Justus Liebig University, Universities of Giessen and Marburg Lung Center, Member of the German Center for Lung Research, Giessen, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
Loading

Abstract

Introduction: Endocytosis plays a pivotal role in cellular and tissue homeostasis. In the lungs, endocytic mechanisms are involved e.g. in phagocytosis, viral infections and receptor-mediated protein uptake. However, the precise lung cell-specific molecular patterns of endocytosis remain poorly understood. Here, we investigated endocytosis in various lung cell types in precision cut lung slices (PCLS), a viable and fully functional 3D lung tissue model system.

Results: By using fluorescent-activated cell sorting (FACS) and confocal microscopy, we studied uptake of different proteins and influenza virus (IV) in PCLS. Our results showed that uptake of albumin, transferrin and IV in epithelial (EpCAM+CD31-CD45-), endothelial (EpCAM-CD31+CD45-) and hematopoietic (EpCAM-CD31-CD45+) cells of PCLS was time- and dose-dependent and of different velocity. Treatment with the endocytosis inhibitor, dynasore, and chlorpromazine, an inhibitor of clathrin-mediated endocytic processes, led to decreased uptake of the cargos and IV in a cell-specific manner. Further differences in endocytic capacity were found, when comparing protein and IV uptake in alveolar epithelial type I and type II cells of PCLS. Moreover, we observed that Ras-related proteins, Rab9 and Rab11, and early endosome antigen-1 (EEA1) played a central role in the uptake of proteins and IV in alveolar epithelial cells from PCLS.

Conclusions: Our studies suggest that PCLS might serve as a novel 3D-model for studying endocytic mechanisms in respiratory cells. Understanding the molecular pathways of endocytosis in the lung may provide new therapeutic approaches for patients with pulmonary diseases.

  • Viruses
  • Epithelial cell
  • Monocyte / Macrophage

Footnotes

Cite this article as: European Respiratory Journal 2021; 58: Suppl. 65, PA3698.

This abstract was presented at the 2021 ERS International Congress, in session “Prediction of exacerbations in patients with COPD”.

This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).

  • Copyright ©the authors 2021
Previous
Back to top
Vol 58 Issue suppl 65 Table of Contents
  • Table of Contents
  • Index by author
Email

Thank you for your interest in spreading the word on European Respiratory Society .

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Murine precision cut lung slices as a novel 3D-model for studying endocytic pathways in the lung
(Your Name) has sent you a message from European Respiratory Society
(Your Name) thought you would like to see the European Respiratory Society web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Murine precision cut lung slices as a novel 3D-model for studying endocytic pathways in the lung
Vitalii Kryvenko, Andrés Alberro Brage, Werner Seeger, Susanne Herold, Christos Samakovlis, István Vadász
European Respiratory Journal Sep 2021, 58 (suppl 65) PA3698; DOI: 10.1183/13993003.congress-2021.PA3698

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Murine precision cut lung slices as a novel 3D-model for studying endocytic pathways in the lung
Vitalii Kryvenko, Andrés Alberro Brage, Werner Seeger, Susanne Herold, Christos Samakovlis, István Vadász
European Respiratory Journal Sep 2021, 58 (suppl 65) PA3698; DOI: 10.1183/13993003.congress-2021.PA3698
del.icio.us logo Digg logo Reddit logo Technorati logo Twitter logo CiteULike logo Connotea logo Facebook logo Google logo Mendeley logo

Jump To

  • Article
  • Info & Metrics
  • Tweet Widget
  • Facebook Like
  • Google Plus One

More in this TOC Section

  • Generating senescent airway epithelial cell populations using low-concentration doxorubicin or etoposide
  • LSC - 2021 - Role of mitochondrial function of lung mesenchymal stem cells in idiopathic pulmonary fibrosis
  • LSC - 2021 - 3D printing aids simultaneous isolation of proximal and distal lung epithelial progenitors from individual mice
Show more Airway cell biology and immunopathology

Related Articles

Navigate

  • Home
  • Current issue
  • Archive

About the ERJ

  • Journal information
  • Editorial board
  • Reviewers
  • Press
  • Permissions and reprints
  • Advertising

The European Respiratory Society

  • Society home
  • myERS
  • Privacy policy
  • Accessibility

ERS publications

  • European Respiratory Journal
  • ERJ Open Research
  • European Respiratory Review
  • Breathe
  • ERS books online
  • ERS Bookshop

Help

  • Feedback

For authors

  • Instructions for authors
  • Publication ethics and malpractice
  • Submit a manuscript

For readers

  • Alerts
  • Subjects
  • Podcasts
  • RSS

Subscriptions

  • Accessing the ERS publications

Contact us

European Respiratory Society
442 Glossop Road
Sheffield S10 2PX
United Kingdom
Tel: +44 114 2672860
Email: journals@ersnet.org

ISSN

Print ISSN:  0903-1936
Online ISSN: 1399-3003

Copyright © 2022 by the European Respiratory Society