Abstract
In this study we aimed to determine the effect of the exosomes derived from Mesenchymal stromal cells (MSC) on cell proliferation, and to study how pre-conditioning the MSCs to a septic environment, changes their exosomes’ content and consequently, their regenerative capacity.
MSC were isolated from male rats’ femora and tibiae and cultured in non pre-stimulated and pre-stimulated (with LPS) conditions. The secreted exosomes were obtained via standard ultracentrifugation protocol. Cell proliferation experiments were conducted by treating an epithelial cell line with exosomes from non pre-stimulated MSC (Control exosomes) and pre-stimulated MSC (LPS exosomes) through an in vitro scratch assay. Exosomal protein profile was analyzed by a liquid chromatography-mass spectrometry analysis.
The treatment of epithelial cells with LPS exosomes increases a 10% its capacity to proliferate and to heal the wound in comparison with the cells treated with Control exosomes (Fig.1a). The analysis of the protein content of both types of exosomes confirmed that pre-conditioning MSCs to a septic environment modifies the protein cargo of its exosomes (Fig. 1b), resulting in the appearance proteins related to cell cycle regulation and cell proliferation.
In conclusion, pre-stimulating MSC with LPS enhances their paracrine activity and improves the reparative capacity of their secreted exosomes.
Footnotes
Cite this article as: European Respiratory Journal 2021; 58: Suppl. 65, PA3617.
This abstract was presented at the 2021 ERS International Congress, in session “Prediction of exacerbations in patients with COPD”.
This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).
- Copyright ©the authors 2021
