Tissue lesions in rat lungs caused by Crotalus durissus collilineatus venom

Abstract

Tissue lesions in rat lungs caused by Crotalus durissus collilineatus venom In 2009, the World Health Organization (WHO) in the list of Neglected Tropical Diseases included snakebite. In most cases, these accidents affect low-income populations living in rural areas, with little or no access to health services. Specimens of the subfamily Crotalinae constitute a group of species that have a wide geographical distribution, occurring in the regions of the Americas and Eurasia. Snakes of this genus have habitats all over Brazil and are responsible for about 8% of the ophidic accidents, reaching a lethality rate of 1.7% (Mcdiarmid, 1999; Bertolozzi, 2015). Due to these data, it is important to understand better the action of the poison in the organism so that it can develop better treatment protocols for this disease. This study investigated lung histology in an experimental model of acute lung injury induced by the effects of Crotalus durissus collilineatus (Cdc) venom on the lungs of male Wistar rats. The animals were divided into four groups and the crude poison, as well as the control, were inoculated intramuscularly into the cranial musculature of the right pelvic limb, with the C (Control) being inoculated with 1µL / g of 0.9% isotonic saline, while in groups V30 '(Poison 30 Minutes), V2 (Poison 2 Hours) and V3 (Poison 3 Hours) the lethal dose of 1µg / g of body weight was injected. After collecting the organs, histological analysis of the lungs was performed, being possible to observe the presence of vascular dilation, hemorrhagic thrombi, and xanthomized macrophages in alveoli. Such results demonstrate that the crude Cdc poison induced histopathological changes in the studied lungs.