Submicroscopic changes of of hemomicrocirculatory bed of the lungs in long term experimental streptozotocin-induced diabetes mellitus

Abstract

Objective: To study the dynamics of ultrastructural changes in the hemocapillaries of the alveolar wall of the lungs in the long term experimental streptozotocin-induced diabetes mellitus.

Methods: Experiments were carried out on 30 white male Wistar rats, 170-210 grams weight. Diabetes mellitus was induced by intraperitoneal injection of streptozotocin solution ('Sigma', USA), diluted in 0.1 M citrate buffer with pH 4,5 in dose of 60 mg/kg of weight. Pulmonary tissue sample acquirement for electronic microscopic research was performed under thiopental narcosis after 42, 56 and 70 days after streptozotocin injection. Sections received on ultramicrotome “Tesla BS-490” were studied using electron microscope “PEM-125K”.

Results: Endothelial cell nuclei with low electron-optical density matrix and marginal aggregation of chromatin granules. The perinuclear space was expanded. In many endothelial cells, the destruction of mitochondria was determined. Cisterns of Goldzhi apparatus alongside with rough endoplasmic reticulum were expanded and fragmentated in most cases. The number of ribosomes on the outer membrane surface of rough endoplasmic reticulum was reduced. The basement membrane underwent thickening with irregular contours. In the peripheral parts of endotheliocytes multiple micropinocytotic vesicles and vacuoles were observed. In the lumen of many hemocapillaries of the alveolar wall, red blood cells sludges and platelet aggregates were noted.

Conclusions: conducted study showed that experimental diabetes mellitus was accompanied by significant dystrophic-destructive changes in the hemomicrocirculatory bed of the alveolar wall in distant terms(70 days).