Abstract
Background: Tuberculous pleural effusion (TPE) is one of the most common forms of extrapulmonary tuberculosis. Patients with tuberculous or malignant pleural effusions (MPE) frequently have similar lymphocytic pleural fluid profile.
Objective: We aimed to determine whether T helper (Th) subsets able to differentiates TPE from non-TPE patients.
Methods: 30 patients with TPE, 30 patients with MPE, 14 patients with EMP and 14 patients with PPE were enrolled between Dec 2018-2019, Tehran-Iran. 3 ml fresh PE harvested and after staining with related Abs, the frequency of T cells phenotypes; CD4+IL-9+, CD4+IL-22+, CD+IL-17+ and CD4+CD25+FOXP3+T cells were determined by flowcytometry.
Results: D4+CD25+FOXP3+ T cells were detected as important markers for TPE (P<0.0001). There were a significant differences in of CD4+IL-9+ T cells in TPE in compared to non-TPE (P<0.0001). But, the frequency of CD+IL-17+ and CD4+IL-22+ T cells had no significant difference between TPE and non-TPE patients (P=0.0906 and P=0.2188, respectively).
Conclusion: The current data suggests that high frequency of CD4+CD25+FOXP3+ T cells may differentiates TPE from non-TPE and future studies with multicenter studies is needed for generalize this finding as a biomarkers.
| T cell subtypes | TPE | Non-TPE | P value |
| CD4+IL-9+ (%) |
3.67 ( 0.87 – 47.83) | 13.05 (1.67 – 61.45) | < 0.0001 |
| CD4+IL-17+ (%) | 7.15(1.118– 9.43) | 15.05 (1.195 -66.55) | 0.0906 |
| CD4+IL-22+ (%) | 0.2 (0.185 – 61.75) | 11.8 (1.119 – 70.5) | 0.2188 |
| CD4+CD25+FOXP3+ (%) | 4.2 (0.362-17.24) | 26.3 (3.349 – 76.93) | <0.0001 |
Footnotes
Cite this article as: European Respiratory Journal 2021; 58: Suppl. 65, PA3089.
This abstract was presented at the 2021 ERS International Congress, in session “Prediction of exacerbations in patients with COPD”.
This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).
- Copyright ©the authors 2021
