Abstract
The phenotype of the disease and the asthma severity can be affected by a genetically mediated level of cytokine concentration. The aim of the work was to study the plasma level of the IL-12B and IL-13 and the distribution of the IL12B rs3212220 and IL13 rs1800925 genotypes.
Methods: Children with asthma were studied: mild severity (n=133), moderate (n=102) and severe (n=82). Control group was represented by healthy individuals (n=229). The plasma level of cytokines was measured using the Milliplex Panel (Millipore, USA). Genotyping was carried out by RT-PCR.
Results: The T* allele rs321220 occurs rather less frequently in moderate asthma individuals (OR 0.63 (0.41-0.97), p=0.035), with it having been assumed its protective role to the disease, whereas the G* allele is predisposing to severe asthma. Allele T* rs1800925 IL13 is more common in patients, whatever the disease severity as related to the control (p=0.01). The significant differences of the plasma level IL-12B in the general cohort of patients with asthma, depending on the IL12B rs321220 genotype were revealed. The plasma level of IL-13 is higher in the CT and TT IL13 rs1800925 genotypes, than in carriers of the CC genotype (Fig.1).
Conclusion: We have identified the genetic markers for susceptibility to asthma in Siberian children, i.e. allele G IL12B rs321220 and allele T IL13 rs1800925 are predisposing to severe asthma.
Footnotes
Cite this article as: European Respiratory Journal 2021; 58: Suppl. 65, PA3078.
This abstract was presented at the 2021 ERS International Congress, in session “Prediction of exacerbations in patients with COPD”.
This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).
- Copyright ©the authors 2021
