Abstract
Electronic antibiograms of Mycobacterium tuberculosis using Illumina whole genome sequencers data and Mykrobe software tool predictor V. Tolchkov, Y. Hodzhev, E. Bachiyska, Y. Atanasova, A. Ivanova, S. Yordanova, S. Panaiotov, T. Kantardzhiev,
Department of Microbiology, National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria
Introduction: M. tuberculosis is the causative agent of the tuberculosis. Drug susceptibility testing is performed by phenotypic and molecular tests. Recently whole genome sequencing analysis of M. tuberculosis strains demonstrated that genotyping of drug resistance can be accurately performed. Software tools are developed for investigation of resistance of microorganisms.
Aim of our study is to perform whole genome sequencing on phenotypically confirmed Bulgarian multi-drug resistant (MDR) M. tuberculosis strains, to identify drug resistant on molecular level and to detect the gene mutations causing the resistance using data of Illumina next generation sequencer.
Materials: We sequenced whole genome of 34 MDR M. tuberculosis Bulgarian strains. FASTQ output of sequencers is analyzed for known drug-resistant mutations with help of the database of the software tool predictor Mykrobe.
Results: Resistance and susceptibility to 11 antibiotics of 34 Bulgarian strains of M. tuberculosis is estimated and presented in this report.
Conclusion: Bioinformatics assay gives more detailed information about mechanism of the antibiotic resistance and has not less sensitivity that the standard phenotypic tests.
Acknowledgements: This research was funded by the BULGARIAN NATIONAL SCIENCE FUND (BNSF), grant number DN13/4 - 15.12.2017
Footnotes
Cite this article as: European Respiratory Journal 2021; 58: Suppl. 65, PA2474.
This abstract was presented at the 2021 ERS International Congress, in session “Prediction of exacerbations in patients with COPD”.
This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).
- Copyright ©the authors 2021