Abstract
Introduction: Primary Cilium (PC) is a sensory cell organelle that regulates viability and growth. PC disruption is not reported in mesothelioma.
Aim: To examine the effects of PC shedding with Ammonium Sulfate (AS), Chloral hydrate (CH) and PC extension with Lithium Chloride (LC) on mesothelioma cell viability and growth.
Methods: Mesothelioma cell lines (M14K;epithelioid, MSTO; biphasic), ZL34; sarcomatoid) were used in the study. Viability of cells on fibronectin substratum (1 day) or sphere formation with 50 cells by the hanging drop method over 2 days with 10% FBS RPMI, or with AS:30mM or with CH:4 mM or LC:50 mM was estimated. Sphere sizes were measured by microscopic imaging.
Results: All treatments significantly reduced viability (p<0.001) and 3D growth compared to respective control treatments normalized to 100%. AS treatments lowered viability (M14K;69±2.6%, MSTO;70±1.8%, ZL34; 65±1.6%) as did CH treatments (M14K; 65±1.9%, MSTO; 53±0.8%, ZL34;54±1.7%). LC lowered viability to M14K; 78±0.9%, MSTO; 68±1.7%, ZL34; 70±2.4%. With AS no spheroids formed in M14K, MSTO while perimeter was lowered in ZL34 (76%, p<0.001) in AS. With CH spheroids were significantly smaller (M14K; 60±6%, ZL34; 62±3.7% both p<0.001, MSTO; 70±7.1%, p<0.05). With LC (M14K; 63.5±6.1%, ZL34; 79.3±3.7% both p<0.001, MSTO; 69.4±2.8%, p<0.01) spheroids growth was tended to be smaller.
Conclusions: The viability of mesothelioma cells and growth in 3D was significantly reduced due to PC perturbation. Loss of growth irrespective of 2D or 3D culture model is suggestive of native PC length requirement for replicative activities in mesothelioma cells.
Footnotes
Cite this article as: European Respiratory Journal 2021; 58: Suppl. 65, OA1521.
This abstract was presented at the 2021 ERS International Congress, in session “Prediction of exacerbations in patients with COPD”.
This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).
- Copyright ©the authors 2021