Supplementary Materials
Additional Files
Supplementary tables
Supplementary tables
- ERJ-00221-2020.Table_S1.xlsx -
Supplementary table S1. List of all the proteins, their Log2FC with respect to day 1 and corresponding fdr values. These data are from mass spec analysis of unstimulated hPCLS over time (day 1, 4, 7, 10 and 13) in ex vivo culture.
- ERJ-00221-2020.Table_S1a.xlsx -
Supplementary table S1a. List of all the proteins that were significantly regulated on day 13 compared to day 1, their Log2FC with respect to day 1 on different days (4, 7, 10 and 13) and corresponding fdr values. These data are from mass spec analysis of unstimulated hPCLS over time (day 1, 4, 7, 10 and 13) in ex vivo culture.
- ERJ-00221-2020.Table_S1b.xlsx -
Supplementary table S1b. List of all the collagens and MMPs that were detected from day 1, 4, 7, 10 and 13, their Log2FC with respect to day 1 on different days (4, 7, 10 and 13) and corresponding fdr values. These data are from mass spec analysis of unstimulated hPCLS over time (day 1, 4, 7, 10 and 13) in ex vivo culture.
- ERJ-00221-2020.Table_S2.xlsx -
Supplementary table S2. List of all the metabolites detected over time in unstimulated hPCLS. The list contains Log2FC with respect to day 1 on different days (4, 7, 10 and 13) and corresponding fdr values. These data are from the metabolomic analysis of the hPCLS.
- ERJ-00221-2020.Table_S2a.xlsx -
Supplementary table S2a. List of all the annotated metabolites detected over time in unstimulated hPCLS. The list contains Log2FC with respect to day 1 on different days (4, 7, 10 and 13) and corresponding fdr values. These data are from the metabolomic analysis of the hPC
- ERJ-00221-2020.Table_S3.xlsx -
Supplementary table S3. List of all the cell-type specific proteins detected, their Log2FC with respect to day 1 and corresponding fdr values. These data are from mass spec analysis of unstimulated hPCLS over time (day 1, 4, 7, 10 and 13) in ex vivo culture that were compared to LGEA database.
- ERJ-00221-2020.Table_S3a.xlsx -
Supplementary table S3a. List of all the cell-type specific proteins detected, that were significantly regulated on day 13 compared to day 1, their Log2FC with respect to day 1 and corresponding fdr values. These data are from mass spec analysis of unstimulated hPCLS over time (day 1, 4, 7, 10 and 13) in ex vivo culture that were compared to LGEA database.
- ERJ-00221-2020.Table_S4.xlsx -
Supplementary table S4. List of all the proteins that were detected on day 13 in vehicle and TGFβ1 treated hPCLS. Their Log2FC with respect to vehicle-treated day 13 hPCLS. These data are from mass spec analysis of vehicle and TGFβ1 treated hPCLS (day 13) in ex vivo culture.
- ERJ-00221-2020.Table_S4a.xlsx -
Supplementary table S4a. List of all the proteins that were significantly regulated in TGFβ1 treated hPCLS compared to vehicle-treated hPCLS. These data are from mass spec analysis of vehicle and TGFβ1 treated hPCLS (day 13) in ex vivo culture.
- ERJ-00221-2020.Table_S5.xlsx -
Supplementary table S5. List of all the cell-type specific proteins detected, their Log2FC with respect to vehicle-treated hPCLS (day 13) and corresponding fdr values. These data are from mass spec analysis of vehicle and TGFβ1 treated hPCLS (day 13) in ex vivo culture and were compared to LGEA database.
- ERJ-00221-2020.Table_S6.xlsx -
Supplementary table S6. These data are from time-course mass spec analysis. Here all the proteins detected are listed. The list is broken into values of each donor and replicate. For example: Day04_F-S-LT21_rep1, refers to changes on day 4 with respect to day 1, in a female (F) donor, that has active smoker status (S), is known as ID LT21 and belongs to replicate. These values are not Log2 transformed, hence a value of 1 means no change, below 1 means a decrease and above 1 means increase with respect to day 1 unstimulated hPCLS.
- ERJ-00221-2020.Table_S7.xlsx -
Supplementary table S7. These data are from vehicle and TGFβ1 treated hPCLS (day 13) mass spec analysis. Here all the proteins detected are listed (NA means not detected in that donor or replicate). The list is broken into values of each donor and replicate. For example: Day04_F-S-LT21_rep1, refers to changes on day 4 with respect to day 1, in a female (F) donor, that has active smoker status (S), is known as ID LT21 and belongs to replicate. These values are not Log2 transformed, hence a value of 1 means no change, below 1 means a decrease and above 1 means increase with respect to day 13 vehicle-treated hPCLS.
- ERJ-00221-2020.Table_S8.xlsx -
Supplementary table S8. Raw values of ELISA measurements of each hPCLS per donor.
- ERJ-00221-2020.Table_S9.xlsx -
Supplementary table S9. Raw values of ELISA measurements of each hPCLS per donor upon vehicle or TGFβ1 stimulation.
- ERJ-00221-2020.Table_S10.xlsx -
Supplementary table S10. raw values of ELISA measurements of each hPCLS per donor upon vehicle, TGFβ1, MMPi and TGFβ1+MMPi stimulation.
- ERJ-00221-2020.Supplement.zip -
FIJI code for interstitial and nor-interstitial ROI selection
- ERJ-00221-2020.Table_S1.xlsx -