Abstract
Background: Recent reports have reported diagnostic and pathogenic relevance of circulating monocytes in interstitial pneumonia.
Objective: We determined whether the expression of M1 and M2 markers on circulating monocytes could be helpful as an adjunct to the diagnoses of idiopathic interstitial pneumonia.
Methods: Forty-seven patients with idiopathic interstitial pneumonia (n = 22 and 25 in idiopathic pulmonary fibrosis [IPF] and idiopathic nonspecific interstitial pneumonia [iNSIP], respectively), and non-smoker healthy volunteers (n = 19) were enrolled in the present study. The expression of the two cell surface markers, S100A9—M1-related and CD163—M2 markers, on circulating monocytes was analyzed by flowcytometer.
Results: The rates of S100A9+cells and S100A9+CD163-cells, and the ratio of S100A9+CD163-cells/S100A9-CD163+ cells in classical monocytes were significantly increased in IPF relative to iNSIP. In contrast, the rates of S100A9-CD163+cells in classical monocytes were significantly decreased in IPF relative to non-smoker healthy volunteers and iNSIP. The results of multivariate regression analyses revealed the independent association of S100A9+CD163-cells in classical monocytes with the differentiation between IPF and iNSIP. The rate of S100A9+CD163-cells in classical monocytes was correlated with serum SP-D levels in IPF. Furthermore, the rate of S100A9+CD163-cells in classical monocytes yielded a receiver operating characteristic-area under the curve of 0.774 for the diagnosis of IPF.
Conclusion: We demonstrate the distinctive M1 polarization in classical circulating monocytes in IPF.
Footnotes
Cite this article as: European Respiratory Journal 2020; 56: Suppl. 64, 723.
This abstract was presented at the 2020 ERS International Congress, in session “Respiratory viruses in the "pre COVID-19" era”.
This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).
- Copyright ©the authors 2020