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Late Breaking Abstract - Biofilm formation by Pseudomonas aeruginosa in empyema

Jinhua Liang, Zhenqiang Zhang, Jinlong Li, Jinliang Kong, Wang Ke
European Respiratory Journal 2020 56: 4666; DOI: 10.1183/13993003.congress-2020.4666
Jinhua Liang
The Department of pulmonary and critical care medicine ,The first affiliated hospital of Guangxi Medical University, Nan ning, China
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Zhenqiang Zhang
The Department of pulmonary and critical care medicine ,The first affiliated hospital of Guangxi Medical University, Nan ning, China
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Jinlong Li
The Department of pulmonary and critical care medicine ,The first affiliated hospital of Guangxi Medical University, Nan ning, China
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Jinliang Kong
The Department of pulmonary and critical care medicine ,The first affiliated hospital of Guangxi Medical University, Nan ning, China
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Wang Ke
The Department of pulmonary and critical care medicine ,The first affiliated hospital of Guangxi Medical University, Nan ning, China
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Abstract

Background: Whether there is biofilm (BF) formation in the pleural cavity of empyema is still inconclusive.

Objectives: We assumed that there is BF formation in empyema, tried to verify this hypothesis.

Methods: We established a model of rabbit empyema infected by Pseudomonas aeruginosa (P.a), and collected pleural tissue and pustule-like specimens. Scanning electron microscope (SEM) was used to observe the P.a-BF on the surface of the pleura. Peptide nucleic acid Fluorescence in situ hybridization (PNA-FISH) was used to observe the P.a-BF in the pus floc.

Results: There were BF-like structures on the surface of the pleura observed by SEM (Fig. 1A), P.a-BF in the pus of pleural cavity confirmed by colony counting and PNA-FISH (Fig. 1B).

Conclusions: To our knowledge, this was the first experiment to confirm the P.a-BF formation in the pleural cavity of empyema, and it was also a novel animal model.

Fig 1A. Biofilm-like structures were observed using SEM at a magnification of 5,000× operating at 30.0 Kv.

Fig1B. Fluorescence microscope image showing P.a-BF surrounded by host cell components. The P.a were stained with a Texas-Red-labeled P.aeruginosa-specific PNA-FISH probe ( red ), and the host cells were stained with DAPI ( blue ).

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  • Inflammation
  • Pleura
  • Bacteria

Footnotes

Cite this article as: European Respiratory Journal 2020; 56: Suppl. 64, 4666.

This abstract was presented at the 2020 ERS International Congress, in session “Respiratory viruses in the "pre COVID-19" era”.

This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).

  • Copyright ©the authors 2020
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Late Breaking Abstract - Biofilm formation by Pseudomonas aeruginosa in empyema
Jinhua Liang, Zhenqiang Zhang, Jinlong Li, Jinliang Kong, Wang Ke
European Respiratory Journal Sep 2020, 56 (suppl 64) 4666; DOI: 10.1183/13993003.congress-2020.4666

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Late Breaking Abstract - Biofilm formation by Pseudomonas aeruginosa in empyema
Jinhua Liang, Zhenqiang Zhang, Jinlong Li, Jinliang Kong, Wang Ke
European Respiratory Journal Sep 2020, 56 (suppl 64) 4666; DOI: 10.1183/13993003.congress-2020.4666
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