Skip to main content

Main menu

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • Open access
    • COVID-19 submission information
    • Peer reviewer login
  • Alerts
  • Podcasts
  • Subscriptions
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

User menu

  • Log in
  • Subscribe
  • Contact Us
  • My Cart

Search

  • Advanced search
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

Login

European Respiratory Society

Advanced Search

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • Open access
    • COVID-19 submission information
    • Peer reviewer login
  • Alerts
  • Podcasts
  • Subscriptions

Alteration of Plasma Exosome Content in Acute Respiratory Distress Syndrome: a miRNA signature associated to lung fibrosing process

Gilles Parzibut, Julien Guiot, Benoit Misset, Michel Malaise, Renaud Louis, Edouard Louis, Monique Henket, Catherine Moermans, Sebastien Njock
European Respiratory Journal 2020 56: 3828; DOI: 10.1183/13993003.congress-2020.3828
Gilles Parzibut
CHU liège, Liege, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: g.parzibut@gmail.com
Julien Guiot
CHU liège, Liege, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Benoit Misset
CHU liège, Liege, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michel Malaise
CHU liège, Liege, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Renaud Louis
CHU liège, Liege, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Edouard Louis
CHU liège, Liege, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Monique Henket
CHU liège, Liege, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Catherine Moermans
CHU liège, Liege, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sebastien Njock
CHU liège, Liege, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
Loading

Abstract

Background: As diagnosis of Acute respiratory distress syndrome (ARDS) is complex, we need to develop novel molecular biomarkers for its detection. In this study, we aimed to characterize microRNA (miRNA) content of exosomes from plasma of ARDS patients and identify altered miRNAs associated to ARDS.

Methods: Exosomes were isolated from plasma of ARDS patients (n=16) and healthy donors (HD) (n=16) via standard ultracentrifugation protocol. The exosomal miRNA profiling was performed using small RNA-Sequencing technology (cohort 1: ARDS (n=7) vs HD (n=7)), and differentially expressed miRNAs were validated with quantitative RT-PCR (cohort 2: ARDS (n=9) vs HD (n=9)). Disease prediction/pathway analysis of validated miRNAs were performed using Ingenuity Pathway Analysis (IPA).

Results: Small RNA-Sequencing analysis has enabled us to identify a substantial dysregulation of plasma exosomal miRNA levels in patients with ARDS. Indeed, 9 miRNAs were differentially expressed in plasma-derived exosomes of ARDS patients compared to HD, among which 7 upregulated and 2 downregulated miRNAs. Using quantitative RT-PCR, we validated the increase of 5 plasma exosomal miRNAs (let-7b-3p, miR-233-3p, miR-24-3p, miR-23a-3p and miR-142-5p) in ARDS context. The bioinformatic analysis by IPA demonstrated that the dysregulated miRNAs are involved in the development of inflammatory disease and lung fibrosis.

Conclusion: For the first time, we identified potential biomarkers for ARDS from plasma-derived exosomes. These candidates should be investigated in large independent cohorts to confirm their biomarker status in ARDS.

  • Pneumonia
  • Acute respiratory failure
  • ARDS (Acute Respiratory Distress Syndrome)

Footnotes

Cite this article as: European Respiratory Journal 2020; 56: Suppl. 64, 3828.

This abstract was presented at the 2020 ERS International Congress, in session “Respiratory viruses in the "pre COVID-19" era”.

This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).

  • Copyright ©the authors 2020
Previous
Back to top
Vol 56 Issue suppl 64 Table of Contents
  • Table of Contents
  • Index by author
Email

Thank you for your interest in spreading the word on European Respiratory Society .

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Alteration of Plasma Exosome Content in Acute Respiratory Distress Syndrome: a miRNA signature associated to lung fibrosing process
(Your Name) has sent you a message from European Respiratory Society
(Your Name) thought you would like to see the European Respiratory Society web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Alteration of Plasma Exosome Content in Acute Respiratory Distress Syndrome: a miRNA signature associated to lung fibrosing process
Gilles Parzibut, Julien Guiot, Benoit Misset, Michel Malaise, Renaud Louis, Edouard Louis, Monique Henket, Catherine Moermans, Sebastien Njock
European Respiratory Journal Sep 2020, 56 (suppl 64) 3828; DOI: 10.1183/13993003.congress-2020.3828

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Alteration of Plasma Exosome Content in Acute Respiratory Distress Syndrome: a miRNA signature associated to lung fibrosing process
Gilles Parzibut, Julien Guiot, Benoit Misset, Michel Malaise, Renaud Louis, Edouard Louis, Monique Henket, Catherine Moermans, Sebastien Njock
European Respiratory Journal Sep 2020, 56 (suppl 64) 3828; DOI: 10.1183/13993003.congress-2020.3828
del.icio.us logo Digg logo Reddit logo Technorati logo Twitter logo CiteULike logo Connotea logo Facebook logo Google logo Mendeley logo

Jump To

  • Article
  • Info & Metrics
  • Tweet Widget
  • Facebook Like
  • Google Plus One

More in this TOC Section

  • Differentiation of NSCLC patients and healthy controls by a urinary microRNA panel
  • LSC - 2020 - Transcriptome signatures of tobacco carcinogens hint the alteration of TAF6 as a specific feature in smokers lung cancer
  • Impact of histaminergic H3 receptor antagonist on hypoglossal nucleus in chronic intermittent hypoxia conditions
Show more Molecular pathology and funct. genomics

Related Articles

Navigate

  • Home
  • Current issue
  • Archive

About the ERJ

  • Journal information
  • Editorial board
  • Reviewers
  • Press
  • Permissions and reprints
  • Advertising

The European Respiratory Society

  • Society home
  • myERS
  • Privacy policy
  • Accessibility

ERS publications

  • European Respiratory Journal
  • ERJ Open Research
  • European Respiratory Review
  • Breathe
  • ERS books online
  • ERS Bookshop

Help

  • Feedback

For authors

  • Instructions for authors
  • Publication ethics and malpractice
  • Submit a manuscript

For readers

  • Alerts
  • Subjects
  • Podcasts
  • RSS

Subscriptions

  • Accessing the ERS publications

Contact us

European Respiratory Society
442 Glossop Road
Sheffield S10 2PX
United Kingdom
Tel: +44 114 2672860
Email: journals@ersnet.org

ISSN

Print ISSN:  0903-1936
Online ISSN: 1399-3003

Copyright © 2022 by the European Respiratory Society