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Late Breaking Abstract - Proteomic changes in respiratory tract lining fluid associated with smoking

Spela Kokelj, Jörgen Östling, Benjamin Georgi, Henric K. Olsson, Anna-Carin Olin
European Respiratory Journal 2020 56: 323; DOI: 10.1183/13993003.congress-2020.323
Spela Kokelj
1University of Gothenburg, Gothenburg, Sweden
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  • For correspondence: spela.kokelj@amm.gu.se
Jörgen Östling
2PExA AB, Gothenburg, Sweden
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Benjamin Georgi
3AstraZeneca, Gothenburg, Sweden
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Henric K. Olsson
3AstraZeneca, Gothenburg, Sweden
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Anna-Carin Olin
1University of Gothenburg, Gothenburg, Sweden
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Abstract

Smoking is the main risk factor for chronic obstructive pulmonary disease (COPD). The aim of this study was to identify dysregulation of specific proteins and pathways associated with smoking in samples from small airways obtained non-invasively with PExA method. 21 healthy controls, 48 ex-smokers and 38 current smokers were examined with the PExA method. 120 ng of samples were collected from individual subjects and analysed with the SOMAscan proteomics platform (SomaLogic Inc). Statistical analyses were performed using Qlucore Omics Explorer and pathway enrichment analysis was performed using the tool Gorilla. 203 proteins were detected in at least 50% of the total set of 107 samples. Smoking exerted a significant impact on the composition of respiratory tract lining fluid (RTLF), with 112 proteins altered due to smoking (p < 0.05, pFDR < 0.089). One of the proteins whose abundance was significantly lower in current smokers compared to ex- and never-smokers was sRAGE (soluble receptor for advanced glycation end products). Gene set enrichment analysis (GSEA) showed that proteins altered by smoking were mainly associated with the vesicle-mediated transport pathway, the protein activation cascade pathway with emphasis on complement activation, and enriched in extracellular exosomes. Our data show the PExA sampling is an efficient tool for studying surfactant changes in small airways, especially given its non-invasive nature. We observed changes in sRAGE in RTLF in connection with smoking, a protein that has previously been proposed as a potential biomarker for COPD. Furthermore, we show a significant effect of smoking on proteins associated with exosomes as well as complement activation.

  • Biomarkers
  • COPD - mechanism

Footnotes

Cite this article as: European Respiratory Journal 2020; 56: Suppl. 64, 323.

This abstract was presented at the 2020 ERS International Congress, in session “Respiratory viruses in the "pre COVID-19" era”.

This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).

  • Copyright ©the authors 2020
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Late Breaking Abstract - Proteomic changes in respiratory tract lining fluid associated with smoking
Spela Kokelj, Jörgen Östling, Benjamin Georgi, Henric K. Olsson, Anna-Carin Olin
European Respiratory Journal Sep 2020, 56 (suppl 64) 323; DOI: 10.1183/13993003.congress-2020.323

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Late Breaking Abstract - Proteomic changes in respiratory tract lining fluid associated with smoking
Spela Kokelj, Jörgen Östling, Benjamin Georgi, Henric K. Olsson, Anna-Carin Olin
European Respiratory Journal Sep 2020, 56 (suppl 64) 323; DOI: 10.1183/13993003.congress-2020.323
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