FIGURE 1 Exposure to toluene 2,4-diisocyanate (TDI) induces airway inflammation. Wild-type (WT) mice were exposed to acetone and olive oil (AOO)/AOO, AOO/TDI or TDI/TDI. a) Exposure protocol, b) total bronchoalveolar lavage (BAL) cells and c) eosinophils; b and c were determined by cytospin. BAL cells were stimulated for 4 h with phorbol 12-myristate 13-acetate/ionomycin+protein transport inhibitors, intracellular labelled and analysed using flow cytometry d) CD4+ T-cells (CD45+, CD11c−, CD11b−, CD45R−, CD5+, TCRβ+, CD4+), e) innate lymphoid cells (ILCs) (CD45+, Lin− (CD11c−, CD11b−, CD45R−, CD5−, TCRβ−), CD90+), f) gating strategy, g) interferon (IFN)-γ+ (interleukin (IL)-17−) CD4+ T-cells, IL13+ CD4+ T-cells, IL-17+ (IFN-γ−) CD4+ T-cells and h) IFN-γ expressing ILCs, IL-13+ ILCs and IL-17+ ILCs are shown. Data are representative of three independent experiments. Representative photomicrographs and quantification of i and j) Congo-red stained peribronchial eosinophils and k and l) periodic acid–Schiff-stained mucus-producing goblet cells. Airways with a perimeter of the basement membrane >800 µm and <2000 µm are included. Data are presented as mean±sd. n=8. Scale bars=100 μm. *: p<0.05.