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Variability in the glycoprotein G gene of RSV-A ON1 strains causing bronchiolitis

Alessandra Pierangeli, Carolina Scagnolari, Agnese Viscido, Camilla Bitossi, Massimo Gentile, Antonella Frassanito, Laura Petrarca, Greta Di Mattia, Raffaella Nenna, Fabio Midulla
European Respiratory Journal 2019 54: PA4997; DOI: 10.1183/13993003.congress-2019.PA4997
Alessandra Pierangeli
1Dpt Molecular Medicine Sapienza University, Roma, Italy
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  • For correspondence: alessandra.pierangeli@uniroma1.it
Carolina Scagnolari
1Dpt Molecular Medicine Sapienza University, Roma, Italy
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Agnese Viscido
1Dpt Molecular Medicine Sapienza University, Roma, Italy
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Camilla Bitossi
1Dpt Molecular Medicine Sapienza University, Roma, Italy
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Massimo Gentile
1Dpt Molecular Medicine Sapienza University, Roma, Italy
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Antonella Frassanito
2Dpt Paediatrics Sapienza University, Roma, Italy
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Laura Petrarca
2Dpt Paediatrics Sapienza University, Roma, Italy
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Greta Di Mattia
2Dpt Paediatrics Sapienza University, Roma, Italy
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Raffaella Nenna
2Dpt Paediatrics Sapienza University, Roma, Italy
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Fabio Midulla
2Dpt Paediatrics Sapienza University, Roma, Italy
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Abstract

Background: Respiratory syncytial virus (RSV)-A genotype ON1 caused a less severe bronchiolitis course than the previously circulating RSV-A genotype (Midulla, F et al JID 2018;219:526-534). However, ON1 continued to circulate worldwide and to differentiate in new strains.

Aim: to study the variability of the viral glycoprotein G in relation to clinical severity in bronchiolitis cases.

Methods: From prospectively enrolled previously healthy term infants hospitalized for bronchiolitis in 2012-13 to 2017-18 epidemic seasons, cases positive to RSV-A ON1 only were selected. After sequencing the second half of the G gene of about 70 samples, a phylogenetic analysis was performed. Demographic and clinical data were compared among divergent ON1 clades.

Results: The phylogenetic analysis of the G gene identified genomic variants appearing since 2012; some of them were not maintained in the following seasons. All sequences did not present polymorphisms in the conserved tract of the G protein that binds the cellular receptor and in the heparin-binding domain. Nonetheless, most strains sequenced in the 2017-18 season set well apart from those of the previous epidemic seasons, in two distant branches of the phylogenetic tree. The two more divergent branches were characterized by patterns of aminoacid changes never reported before, some of which located in antigenic sites. Moreover, substitutions may have altered N- and O-glycosilation sites. Patients infected with divergent strains presented higher severity scores.

Conclusions: The aminoacid changes detected in the hypervariable part of the G protein may have altered functions and/or changed its immunogenicity determining an impact on disease severity.

  • Mutations
  • Bronchiolitis
  • Viruses

Footnotes

Cite this article as: European Respiratory Journal 2019; 54: Suppl. 63, PA4997.

This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).

  • Copyright ©the authors 2019
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Variability in the glycoprotein G gene of RSV-A ON1 strains causing bronchiolitis
Alessandra Pierangeli, Carolina Scagnolari, Agnese Viscido, Camilla Bitossi, Massimo Gentile, Antonella Frassanito, Laura Petrarca, Greta Di Mattia, Raffaella Nenna, Fabio Midulla
European Respiratory Journal Sep 2019, 54 (suppl 63) PA4997; DOI: 10.1183/13993003.congress-2019.PA4997

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Variability in the glycoprotein G gene of RSV-A ON1 strains causing bronchiolitis
Alessandra Pierangeli, Carolina Scagnolari, Agnese Viscido, Camilla Bitossi, Massimo Gentile, Antonella Frassanito, Laura Petrarca, Greta Di Mattia, Raffaella Nenna, Fabio Midulla
European Respiratory Journal Sep 2019, 54 (suppl 63) PA4997; DOI: 10.1183/13993003.congress-2019.PA4997
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