Abstract
Background: Nowadays stem cells participation in tumor growth has been extensively discussed in anticancer therapy. Mesenchymal stem cells (MSCs) produced the transient arrest of tumor cells in the G1 phase of cell cycle. However, previous studies have produced results regarding whether MSCs promote or inhibit tumor growth. In view of these controversial data, we conducted a study on the impact of allogeneic MSCs on biological properties of the primary tumor cells of Lewis lung carcinoma.
Methods: The investigation was carried out in C57Bl/6 male mice. Mice of experimental group received the course of inoculation of MSCs. The number of living cells was determined using MTT-colorymetric test. Apoptotic level and distribution of cells in phases of cell cycle were assessed by cytofluorimetry.
Results: MSCs were isolated from primary tumor, incubated 24 hours and then apoptotic level was assessed in control and test samples. The number aneuploid cells increased in 1.3 times upon MSCs influence on transplantable lung Lewis carcinoma. Among aneuploid cells population, increase of cells of proliferative pool were detected after MSCs administration in comparison with control. Decrease of percent of diploid cells subpopulation in 1,7 times in primary culture after MSCs administration and their more than 90% synchronization G0/G1 phase of cells cycle was shown. Regarding aneuploid cells after MSCs influence, increase of this population to 77,02+3,83% versus 59,20+1,71% in control was accompanied by growth of proliferative pool subpopulation.
Conclusion: Thus, biological characteristics of tumor cells under the influence of MSCs are primarily associated with the increase of aneuploidy.
Footnotes
Cite this article as: European Respiratory Journal 2019; 54: Suppl. 63, PA3655.
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- Copyright ©the authors 2019