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Effect of Vaping on Airway Barrier Function: A Pilot Study

Baishakhi Ghosh, Kristine Nishida, Lakshmana Chandrala, Venkataramana Sidhaye
European Respiratory Journal 2019 54: PA2395; DOI: 10.1183/13993003.congress-2019.PA2395
Baishakhi Ghosh
1Department of Environment Health and Enginnering, Johns Hopkins Bloomberg School of Public Health, Baltimore, United States of America
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  • For correspondence: bghosh226@gmail.com
Kristine Nishida
1Department of Environment Health and Enginnering, Johns Hopkins Bloomberg School of Public Health, Baltimore, United States of America
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Lakshmana Chandrala
2Department of Mechanical Engineering, Johns Hopkins Whiting School of Engineering, Baltimore, United States of America
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Venkataramana Sidhaye
3Pulmonary Critical Care Medicine, Johns Hopkins School of Medicine & Department of Environment Health and Enginnering, Johns Hopkins Bloomberg School of Public Health, Baltimore, United States of America
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Abstract

Electronic-cigarettes (ECs) are battery powered devices (containing ~7X1011 free radicals/puff), vaporizes flavors with/without nicotine, which may aid smokers in quitting or attenuating their tobacco habits. To date, health concerns of vaping EC use among healthy & COPD is limited and whether regular use has any effect on airway epithelium. We aim to investigate if vaping alters the structure & function of the airway epithelial cells.

Primary human bronchial epithelia from 5 healthy donors (NHBE, Lonza & Epithelix) were differentiated on an air-liquid interface & exposed to popular EC flavored aerosols (Tobacco/Mango) with/without nicotine compared to clean air control for 10 days using a Vitrocell exposure system connected to a peristaltic pump (CRM81 puffing regimen). Epithelial function was assessed by cell permeability (FITC-dextran assay & TEER), number of cilia, & ciliary beat frequency (CBF). Effects on cell-cell adhesion were measured by quantifying junctional protein abundance & functional effects of altered adhesion were measured by computing cellular motion over time.

We observed that vaping EC aerosols increased permeability, as well as decreased number of cilia & CBF of the airway epithelia (Fig.1). In addition, decreased cell-cell adhesion was quantified by increased cellular motion over time.

Our data suggests that vaping EC disrupts structural and functional integrity of airway epithelia, resulting in airway inflammation & lung diseases.

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Footnotes

Cite this article as: European Respiratory Journal 2019; 54: Suppl. 63, PA2395.

This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).

  • Copyright ©the authors 2019
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Effect of Vaping on Airway Barrier Function: A Pilot Study
Baishakhi Ghosh, Kristine Nishida, Lakshmana Chandrala, Venkataramana Sidhaye
European Respiratory Journal Sep 2019, 54 (suppl 63) PA2395; DOI: 10.1183/13993003.congress-2019.PA2395

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Effect of Vaping on Airway Barrier Function: A Pilot Study
Baishakhi Ghosh, Kristine Nishida, Lakshmana Chandrala, Venkataramana Sidhaye
European Respiratory Journal Sep 2019, 54 (suppl 63) PA2395; DOI: 10.1183/13993003.congress-2019.PA2395
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