Abstract
Background: Interstitial lung disease (ILD) in children, is associated with neutrophil hyperactivity in terms of reactive oxygen species (ROS) generation. ROS over-production can damage surrounding tissues and participate in exaggerated inflammatory processes. We have determined the effect of antioxidant echinochrome (2,3,5,6,8-pentahydroxy-7-ethyl-1,4-naphthaquinone) on blood neutrophil ROS generation in children with ILD in vitro.
Methods: 21 patients with ILD ( median age 9.0±3.1 years) were observed. As a control 20 children of similar age range were examined. Neutrophils’ oxidative metabolism was measured by chemiluminescence (CL). Luminol-dependent CL (CL-lum), allows one to determine phagocytic activity and the level of ROS generated in a myeloperoxidase-dependent manner. Lcigenin-dependent CL (CL-luc) measures the NADPH oxidase-dependent extracellular ROS secretion. Blood neutrophils were incubated with echinochrome prior to stimulation with serum-opsonized zymosan.
Results: In patients with ILD, neutrophils unstimulated and stimulated by opsonized zymosan show an increase (in 2.7- to 5.0-fold, p<0.05) in the level of CL-luc and CL-lum compared to the control group. The presence of echinochrome (100 mkM) decreased CL-luc and CL-lum of resting and zymosan-stimulated neutrophils (p<0.05) in blood of children with ILD and without ILD (control group).
Conclusions: Eechinochrome appears as a promising agent in the prevention of oxidative damage caused by blood neutrophil hyperactivity in children with ILD. The work was supported by the Far East program FEB RAS (project 18-4-037).
Footnotes
Cite this article as: European Respiratory Journal 2019; 54: Suppl. 63, PA2361.
This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).
- Copyright ©the authors 2019