Skip to main content

Main menu

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • For authors
    • Instructions for authors
    • Submit a manuscript
    • Author FAQs
    • Open access
    • COVID-19 submission information
  • Alerts
  • Podcasts
  • Subscriptions
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

User menu

  • Log in
  • Subscribe
  • Contact Us
  • My Cart

Search

  • Advanced search
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

Login

European Respiratory Society

Advanced Search

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • For authors
    • Instructions for authors
    • Submit a manuscript
    • Author FAQs
    • Open access
    • COVID-19 submission information
  • Alerts
  • Podcasts
  • Subscriptions

Intragraft donor-specific anti-HLA antibodies in phenotypes of chronic lung allograft dysfunction

Annelore Sacreas, Jean-Luc Taupin, Marie-Paule Emonds, Liesbeth Daniëls, Dirk E. Van Raemdonck, Robin Vos, Geert M. Verleden, Bart M. Vanaudenaerde, Antoine Roux, Stijn E. Verleden and the Leuven Lung Transplant Group
European Respiratory Journal 2019 54: 1900847; DOI: 10.1183/13993003.00847-2019
Annelore Sacreas
1Leuven Lung Transplant Group, Dept of Chronic Diseases, Metabolism and Ageing (CHROMETA), KU Leuven, Leuven, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jean-Luc Taupin
2Laboratoire d'Immunologie et Histocompatibilité, Saint-Louis Hospital, Paris, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Marie-Paule Emonds
3Histocompatibility and Immunogenetics Laboratory, Belgian Red Cross-Flanders, Mechelen, Belgium
4Dept of Immunology and Microbiology, KU Leuven, Leuven, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Liesbeth Daniëls
3Histocompatibility and Immunogenetics Laboratory, Belgian Red Cross-Flanders, Mechelen, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dirk E. Van Raemdonck
1Leuven Lung Transplant Group, Dept of Chronic Diseases, Metabolism and Ageing (CHROMETA), KU Leuven, Leuven, Belgium
5Dept of Thoracic Surgery, University Hospitals Leuven, Leuven, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Robin Vos
1Leuven Lung Transplant Group, Dept of Chronic Diseases, Metabolism and Ageing (CHROMETA), KU Leuven, Leuven, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Robin Vos
Geert M. Verleden
1Leuven Lung Transplant Group, Dept of Chronic Diseases, Metabolism and Ageing (CHROMETA), KU Leuven, Leuven, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bart M. Vanaudenaerde
1Leuven Lung Transplant Group, Dept of Chronic Diseases, Metabolism and Ageing (CHROMETA), KU Leuven, Leuven, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Antoine Roux
6Service de Transplantation Pulmonaire, Foch Hospital, Suresnes, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stijn E. Verleden
1Leuven Lung Transplant Group, Dept of Chronic Diseases, Metabolism and Ageing (CHROMETA), KU Leuven, Leuven, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: stijn.verleden@kuleuven.be
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Abstract

Introduction Circulating anti-human leukocyte antigen (HLA) serum donor-specific antibodies (sDSAs) increase the risk of chronic lung allograft dysfunction (CLAD) and mortality. Discrepancies between serological and pathological/clinical findings are common. Therefore, we aimed to assess the presence of tissue-bound graft DSAs (gDSAs) in CLAD explant tissue compared with sDSAs.

Methods Tissue cores, obtained from explant lungs of unused donors (n=10) and patients with bronchiolitis obliterans syndrome (BOS; n=18) and restrictive allograft syndrome (RAS; n=18), were scanned with micro-computed tomography before elution of antibodies. Total IgG levels were measured via ELISA. Anti-HLA class I and II IgG gDSAs were identified using Luminex single antigen beads and compared with DSAs found in serum samples.

Results Overall, mean fluorescence intensity was higher in RAS eluates compared with BOS and controls (p<0.0001). In BOS, two patients were sDSA+/gDSA+ and two patients were sDSA−/gDSA+. In RAS, four patients were sDSA+/gDSA+, one patient was sDSA+/gDSA− and five patients were sDSA−/gDSA+. Serum and graft results combined, DSAs were more prevalent in RAS compared with BOS (56% versus 22%; p=0.04). There was spatial variability in gDSA detection in one BOS patient and three RAS patients, who were all sDSA−. Total graft IgG levels were higher in RAS than BOS (p<0.0001) and in gDSA+ versus gDSA− (p=0.0008), but not in sDSA+ versus sDSA− (p=0.33). In RAS, total IgG levels correlated with fibrosis (r= −0.39; p=0.02).

Conclusions This study underlines the potential of gDSA assessment as complementary information to sDSA findings. The relevance and applications of gDSAs need further investigation.

Abstract

Discrepancies between serological and pathological/clinical findings in DSAs after lung transplantation are common. This article provides evidence that graft DSA assessment yields complementary information for lung transplant patients. http://bit.ly/2GIkae1

Footnotes

  • The Leuven Lung Transplant Group includes: Anke Van Herck, Janne Kaes, Tobias Heigl, Sofie Ordies, Laurens J. De Sadeleer, Arno Vanstapel, Arne P. Neyrinck, Veronique Schaevers, Lieven J. Dupont, Jonas Yserbyt, Laurent Godinas, Lieven Depypere, Anna E. Frick, Laurens J. Ceulemans, Eric K. Verbeken, Birgit Weynand, Paul De Leyn, Willy Coosemans, Hans Van Veer, Philippe Nafteux and Herbert Decaluwé.

  • Support statement: R. Vos is senior clinical research fellow of the Research Foundation Flanders (FWO), Belgium (12G8715N) and is supported by a research grant of UZ Leuven, Belgium (STG15/023). S.E. Verleden is a post-doctoral research fellow of the Research Foundation Flanders (FWO 12G8715N and 1500617N), Belgium and is supported by a research grant from the International Society for Heart and Lung Transplantation and the KU Leuven (C24/18/073)

  • Conflict of interest: A. Sacreas has nothing to disclose.

  • Conflict of interest: J-L. Taupin has nothing to disclose.

  • Conflict of interest: M-P. Emonds has nothing to disclose.

  • Conflict of interest: L. Daniëls has nothing to disclose.

  • Conflict of interest: D.E. Van Raemdonck has nothing to disclose.

  • Conflict of interest: R. Vos has nothing to disclose.

  • Conflict of interest: G.M. Verleden has nothing to disclose.

  • Conflict of interest: B.M. Vanaudenaerde has nothing to disclose.

  • Conflict of interest: A. Roux reports personal fees and nonfinancial support from Biotest, personal fees from Thermo Fisher, nonfinancial support from CSL Berhing, outside the submitted work.

  • Conflict of interest: S.E. Verleden has nothing to disclose.

  • Received April 29, 2019.
  • Accepted July 31, 2019.
  • Copyright ©ERS 2019
https://www.ersjournals.com/user-licence
View Full Text

ERS Members

myERS - ERS members : log in with your myERS username and password.

INDIVIDUALS

Log in Login as an individual user.

Forgot your username or password?

LIBRARY USERS

Log in through your institution

If your library has a subscription, you may already be logged in via your IP address. Otherwise you may be able to log in via one of the following routes.
You may be able to gain access using your login credentials for your institution. Contact your library if you do not have a username and password.
If your organization uses OpenAthens, you can log in using your OpenAthens username and password. To check if your institution is supported, please see this list. Contact your library for more details.
If you think you should have access, please contact your librarian or email journals@ersnet.org

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top
View this article with LENS
Vol 54 Issue 5 Table of Contents
European Respiratory Journal: 54 (5)
  • Table of Contents
  • Index by author
Email

Thank you for your interest in spreading the word on European Respiratory Society .

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Intragraft donor-specific anti-HLA antibodies in phenotypes of chronic lung allograft dysfunction
(Your Name) has sent you a message from European Respiratory Society
(Your Name) thought you would like to see the European Respiratory Society web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Print
Alerts
Sign In to Email Alerts with your Email Address
Citation Tools
Intragraft donor-specific anti-HLA antibodies in phenotypes of chronic lung allograft dysfunction
Annelore Sacreas, Jean-Luc Taupin, Marie-Paule Emonds, Liesbeth Daniëls, Dirk E. Van Raemdonck, Robin Vos, Geert M. Verleden, Bart M. Vanaudenaerde, Antoine Roux, Stijn E. Verleden
European Respiratory Journal Nov 2019, 54 (5) 1900847; DOI: 10.1183/13993003.00847-2019

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Intragraft donor-specific anti-HLA antibodies in phenotypes of chronic lung allograft dysfunction
Annelore Sacreas, Jean-Luc Taupin, Marie-Paule Emonds, Liesbeth Daniëls, Dirk E. Van Raemdonck, Robin Vos, Geert M. Verleden, Bart M. Vanaudenaerde, Antoine Roux, Stijn E. Verleden
European Respiratory Journal Nov 2019, 54 (5) 1900847; DOI: 10.1183/13993003.00847-2019
del.icio.us logo Digg logo Reddit logo Technorati logo Twitter logo CiteULike logo Connotea logo Facebook logo Google logo Mendeley logo
Full Text (PDF)

Jump To

  • Article
    • Abstract
    • Abstract
    • Introduction
    • Materials and methods
    • Results
    • Discussion
    • Shareable PDF
    • Acknowledgements
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • PDF

Subjects

  • Interstitial and orphan lung disease
  • Tweet Widget
  • Facebook Like
  • Google Plus One

More in this TOC Section

Original Articles

  • EmPHasis-10 as a measure of quality of life in PAH
  • EmPHasis-10 health-related quality of life score predicts outcomes in PAH
  • Periostin and airway epithelial function in asthma
Show more Original Articles

Lung transplantation

  • P. aeruginosa eradication improves outcomes after lung transplantation
  • Acute fibrinous and organising pneumonia impairs long-term lung allograft function
Show more Lung transplantation

Related Articles

Navigate

  • Home
  • Current issue
  • Archive

About the ERJ

  • Journal information
  • Editorial board
  • Reviewers
  • CME
  • Press
  • Permissions and reprints
  • Advertising

The European Respiratory Society

  • Society home
  • myERS
  • Privacy policy
  • Accessibility

ERS publications

  • European Respiratory Journal
  • ERJ Open Research
  • European Respiratory Review
  • Breathe
  • ERS books online
  • ERS Bookshop

Help

  • Feedback

For authors

  • Instructions for authors
  • Submit a manuscript
  • ERS author centre

For readers

  • Alerts
  • Subjects
  • Podcasts
  • RSS

Subscriptions

  • Accessing the ERS publications

Contact us

European Respiratory Society
442 Glossop Road
Sheffield S10 2PX
United Kingdom
Tel: +44 114 2672860
Email: journals@ersnet.org

ISSN

Print ISSN:  0903-1936
Online ISSN: 1399-3003

Copyright © 2021 by the European Respiratory Society