Skip to main content

Main menu

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • For authors
    • Instructions for authors
    • Submit a manuscript
    • Author FAQs
    • Open access
    • COVID-19 submission information
  • Alerts
  • Podcasts
  • Subscriptions
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

User menu

  • Log in
  • Subscribe
  • Contact Us
  • My Cart

Search

  • Advanced search
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

Login

European Respiratory Society

Advanced Search

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • For authors
    • Instructions for authors
    • Submit a manuscript
    • Author FAQs
    • Open access
    • COVID-19 submission information
  • Alerts
  • Podcasts
  • Subscriptions

Whole-transcriptome sequencing reveals heightened inflammation and defective host defence responses in chronic rhinosinusitis with nasal polyps

Yang Peng, Xiao-Xue Zi, Teng-Fei Tian, Bernett Lee, Josephine Lum, See Aik Tang, Kai Sen Tan, Qian-Hui Qiu, Jing Ye, Li Shi, Wei-Jie Guan, Anand Kumar Andiappan, De Yun Wang
European Respiratory Journal 2019 54: 1900732; DOI: 10.1183/13993003.00732-2019
Yang Peng
1Dept of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
2Dept of Otolaryngology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China
3State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, Guangdong, China
8These authors contributed equally to this work
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xiao-Xue Zi
1Dept of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
4Shandong Provincial ENT Hospital Affiliated to Shandong University, Shandong University, Jinan, China
8These authors contributed equally to this work
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Teng-Fei Tian
1Dept of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
5Dept of Otolaryngology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China
8These authors contributed equally to this work
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bernett Lee
6Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Josephine Lum
6Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
See Aik Tang
6Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kai Sen Tan
1Dept of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Qian-Hui Qiu
2Dept of Otolaryngology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China
7Dept of Otolaryngology Head and Neck Surgery, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jing Ye
5Dept of Otolaryngology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Li Shi
4Shandong Provincial ENT Hospital Affiliated to Shandong University, Shandong University, Jinan, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Wei-Jie Guan
1Dept of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
3State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, Guangdong, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Anand Kumar Andiappan
6Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: anand_andiappan@immunol.a-star.edu.sg battery203@163.com
De Yun Wang
1Dept of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for De Yun Wang
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Abstract

Introduction The pathways underlying chronic rhinosinusitis with nasal polyps (CRSwNP) are unclear. We conducted genome-wide gene expression analysis to determine pathways and candidate gene sets associated with CRSwNP.

Methods We performed whole-transcriptome RNA sequencing on 42 polyp (CRSwNP-NP) and 33 paired nonpolyp inferior turbinate (CRSwNP-IT) tissues from patients with CRSwNP and 28 inferior turbinate samples from non-CRS controls (CS-IT). We analysed the differentially expressed genes (DEGs) and the gene sets that were enriched in functional pathways.

Results Principal component-informed analysis revealed cilium function and immune regulation as the two main Gene Ontology (GO) categories differentiating CRSwNP patients from controls. We detected 6182 and 1592 DEGs between CRSwNP-NP versus CS-IT and between CRSwNP-NP versus CRSwNP-IT tissues, respectively. Atopy status did not have a major impact on gene expression in various tissues. GO analysis on these DEGs implicated extracellular matrix (ECM) disassembly, O-glycan processing, angiogenesis and host viral response in CRSwNP pathogenesis. Ingenuity Pathway Analysis identified significant enrichment of type 1 interferon signalling and axonal guidance canonical pathways, angiogenesis, and collagen and fibrotic changes in CRSwNP (CRSwNP-NP and CRSwNP-IT) tissues compared with CS-IT. Finally, gene set enrichment analysis implicated sets of genes co-regulated in processes associated with inflammatory response and aberrant cell differentiation in polyp formation.

Conclusions Gene signatures involved in defective host defences (including cilia dysfunction and immune dysregulation), inflammation and abnormal metabolism of ECM are implicated in CRSwNP. Functional validation of these gene expression patterns will open opportunities for CRSwNP therapeutic interventions such as biologics and immunomodulators.

Abstract

Whole-transcriptome sequencing shows gene signatures associated with interferon signalling response and host defence are implicated in chronic rhinosinusitis with nasal polyps, opening up new opportunities for therapeutic interventions http://bit.ly/2KyF3dT

Footnotes

  • This article has supplementary material available from erj.ersjournals.com

  • Author contributions: Conceived and designed the experiments: D.Y. Wang, A.K. Andiappan and W-J. Guan. Collection of samples: Y. Peng, X-X. Zi, T-F. Tian, L. Shi, J. Ye and Q-H. Qiu. Performed the experiments: Y. Peng, X-X. Zi, T-F. Tian and A.K. Andiappan. Data analysis: A.K. Andiappan, Y. Peng, X-X. Zi, T-F. Tian, W-J. Guan, B. Lee, J. Lum, S.A. Tang and K.S. Tan. Contributed reagents/materials/analysis tools: A.K. Andiappan and D.Y. Wang. Wrote the paper: W-J. Guan, A.K. Andiappan, K.S. Tan, Y. Peng, X-X. Zi, T-F. Tian. Critical review and approval: A.K. Andiappan, D.Y. Wang and W-J. Guan.

  • Conflict of interest: Y. Peng has nothing to disclose.

  • Conflict of interest: X-X. Zi has nothing to disclose.

  • Conflict of interest: T-F. Tian has nothing to disclose.

  • Conflict of interest: B. Lee has nothing to disclose.

  • Conflict of interest: J. Lum has nothing to disclose.

  • Conflict of interest: S.A. Tang has nothing to disclose.

  • Conflict of interest: K.S. Tan has nothing to disclose.

  • Conflict of interest: Q-H. Qiu has nothing to disclose.

  • Conflict of interest: J. Ye has nothing to disclose.

  • Conflict of interest: L. Shi reports grants from The Key Research Development Program of Shandong Province (2018CXGC1214), outside the submitted work.

  • Conflict of interest: W-J. Guan reports grants from Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme 2017, during the conduct of the study.

  • Conflict of interest: A.K. Andiappan reports grants from National Medical Research Council of Singapore (OFYIRG17nov065), during the conduct of the study.

  • Conflict of interest: D.Y. Wang reports grants from National Medical Research Council of Singapore (NMRC/CIRG/1458/2016), outside the submitted work.

  • Support statement: Supported by National Medical Research Council of Singapore (NMRC/CIRG/1458/2016; D.Y. Wang), National Medical Research Council of Singapore (OFYIRG17nov065; A.K. Andiappan), The Key Research Development Program of Shandong Province (2018CXGC1214; L. Shi), Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme 2017 (201710010097; W-J. Guan), and the National Nature Science Foundation of China (81873690; Q-H. Qiu). Funding information for this article has been deposited with the Crossref Funder Registry.

  • Received April 11, 2019.
  • Accepted August 8, 2019.
  • Copyright ©ERS 2019
https://www.ersjournals.com/user-licence
View Full Text

ERS Members

myERS - ERS members : log in with your myERS username and password.

INDIVIDUALS

Log in Login as an individual user.

Forgot your username or password?

LIBRARY USERS

Log in through your institution

If your library has a subscription, you may already be logged in via your IP address. Otherwise you may be able to log in via one of the following routes.
You may be able to gain access using your login credentials for your institution. Contact your library if you do not have a username and password.
If your organization uses OpenAthens, you can log in using your OpenAthens username and password. To check if your institution is supported, please see this list. Contact your library for more details.
If you think you should have access, please contact your librarian or email journals@ersnet.org

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top
View this article with LENS
Vol 54 Issue 5 Table of Contents
European Respiratory Journal: 54 (5)
  • Table of Contents
  • Index by author
Email

Thank you for your interest in spreading the word on European Respiratory Society .

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Whole-transcriptome sequencing reveals heightened inflammation and defective host defence responses in chronic rhinosinusitis with nasal polyps
(Your Name) has sent you a message from European Respiratory Society
(Your Name) thought you would like to see the European Respiratory Society web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Print
Alerts
Sign In to Email Alerts with your Email Address
Citation Tools
Whole-transcriptome sequencing reveals heightened inflammation and defective host defence responses in chronic rhinosinusitis with nasal polyps
Yang Peng, Xiao-Xue Zi, Teng-Fei Tian, Bernett Lee, Josephine Lum, See Aik Tang, Kai Sen Tan, Qian-Hui Qiu, Jing Ye, Li Shi, Wei-Jie Guan, Anand Kumar Andiappan, De Yun Wang
European Respiratory Journal Nov 2019, 54 (5) 1900732; DOI: 10.1183/13993003.00732-2019

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Whole-transcriptome sequencing reveals heightened inflammation and defective host defence responses in chronic rhinosinusitis with nasal polyps
Yang Peng, Xiao-Xue Zi, Teng-Fei Tian, Bernett Lee, Josephine Lum, See Aik Tang, Kai Sen Tan, Qian-Hui Qiu, Jing Ye, Li Shi, Wei-Jie Guan, Anand Kumar Andiappan, De Yun Wang
European Respiratory Journal Nov 2019, 54 (5) 1900732; DOI: 10.1183/13993003.00732-2019
del.icio.us logo Digg logo Reddit logo Technorati logo Twitter logo CiteULike logo Connotea logo Facebook logo Google logo Mendeley logo
Full Text (PDF)

Jump To

  • Article
    • Abstract
    • Abstract
    • Introduction
    • Methods
    • Results
    • Discussion
    • Supplementary material
    • Shareable PDF
    • Acknowledgements
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • PDF

Subjects

  • Asthma and allergy
  • Tweet Widget
  • Facebook Like
  • Google Plus One

More in this TOC Section

Original Articles

  • EmPHasis-10 as a measure of quality of life in PAH
  • EmPHasis-10 health-related quality of life score predicts outcomes in PAH
  • Periostin and airway epithelial function in asthma
Show more Original Articles

Asthma and allergy

  • Allergic diseases and long-term risk of autoimmune disorders
  • Laboratory animal allergy is preventable
Show more Asthma and allergy

Related Articles

Navigate

  • Home
  • Current issue
  • Archive

About the ERJ

  • Journal information
  • Editorial board
  • Reviewers
  • CME
  • Press
  • Permissions and reprints
  • Advertising

The European Respiratory Society

  • Society home
  • myERS
  • Privacy policy
  • Accessibility

ERS publications

  • European Respiratory Journal
  • ERJ Open Research
  • European Respiratory Review
  • Breathe
  • ERS books online
  • ERS Bookshop

Help

  • Feedback

For authors

  • Instructions for authors
  • Submit a manuscript
  • ERS author centre

For readers

  • Alerts
  • Subjects
  • Podcasts
  • RSS

Subscriptions

  • Accessing the ERS publications

Contact us

European Respiratory Society
442 Glossop Road
Sheffield S10 2PX
United Kingdom
Tel: +44 114 2672860
Email: journals@ersnet.org

ISSN

Print ISSN:  0903-1936
Online ISSN: 1399-3003

Copyright © 2021 by the European Respiratory Society