Abstract
A proportion of patients with fibrosing interstitial lung diseases (ILDs) develop a progressive phenotype characterised by decline in lung function, worsening quality of life and early mortality. Other than idiopathic pulmonary fibrosis (IPF), there are no approved drugs for fibrosing ILDs and a poor evidence base to support current treatments. Fibrosing ILDs with a progressive phenotype show commonalities in clinical behaviour and in the pathogenic mechanisms that drive disease worsening. Nintedanib is an intracellular inhibitor of tyrosine kinases that has been approved for treatment of IPF and has recently been shown to reduce the rate of lung function decline in patients with ILD associated with systemic sclerosis (SSc-ILD). In vitro data demonstrate that nintedanib inhibits several steps in the initiation and progression of lung fibrosis, including the release of pro-inflammatory and pro-fibrotic mediators, migration and differentiation of fibrocytes and fibroblasts, and deposition of extracellular matrix. Nintedanib also inhibits the proliferation of vascular cells. Studies in animal models with features of fibrosing ILDs such as IPF, SSc-ILD, rheumatoid arthritis-ILD, hypersensitivity pneumonitis and silicosis demonstrate that nintedanib has anti-fibrotic activity irrespective of the trigger for the lung pathology. This suggests that nintedanib inhibits fundamental processes in the pathogenesis of fibrosis. A trial of nintedanib in patients with progressive fibrosing ILDs other than IPF (INBUILD) will report results in 2019.
Abstract
Nonclinical studies suggest that nintedanib, an approved treatment for idiopathic pulmonary fibrosis, may have anti-fibrotic effects in other interstitial lung diseases with a progressive fibrosing phenotype http://bit.ly/2NiCAYx
Introduction
Interstitial lung diseases (ILDs) comprise a large and varied group of diseases that generally affect the interstitium, the connective tissue stroma that separates the epithelial and endothelial barriers in the lungs. ILDs may have a known cause, e.g. they may be a manifestation of an autoimmune disease or a result of sensitisation to an inhaled antigen. However, ILDs also include several diseases of unknown cause [1]. Idiopathic pulmonary fibrosis (IPF) is one of the most common types of ILD. Although the course of IPF is variable, IPF is, by definition, a progressive fibrosing disease that ultimately results in respiratory failure [2]. A proportion of patients with certain other types of ILD also develop a progressive fibrosing phenotype associated with decline in lung function, worsening symptoms and quality of life, and early mortality [3]. These include idiopathic nonspecific interstitial pneumonia [4], ILDs associated with autoimmune diseases such as rheumatoid arthritis (RA-ILD) [5] and systemic sclerosis (SSc-ILD) [6], chronic hypersensitivity pneumonitis [7], chronic sarcoidosis [8], exposure-related ILDs such as silicosis [9], and unclassifiable idiopathic interstitial pneumonia [10]. At present, the goal of treatment for fibrosing ILDs with a progressive phenotype is to slow the decline in lung function. However, IPF is the only form of fibrosing ILD for which approved drug therapies are available. The treatment of patients with other types of progressive fibrosing ILD is based largely on immunosuppression, which, other than in SSc-ILD, is not supported by a robust evidence base, leaving patients with a high unmet need for efficacious treatment options [11, 12].
Pathogenesis of fibrosing ILDs with progressive phenotype
Several mechanisms are known to be involved in the pathogenesis and progression of all fibrosing ILDs (figure 1). All ILDs are believed to be triggered by repetitive chronic epithelial or vascular injuries, or by granulomatous inflammation, which lead to cell destruction and, in the case of fibrotic disease, to unregulated repair [13–15]. Fibroblasts, the main effector cells in fibrosing ILDs, are drawn from different sources to the site of injury. Resident interstitial fibroblasts proliferate and migrate [16], fibrocytes from the circulation invade [17], and epithelial cells [18] and pericytes [19] acquire features usually associated with fibroblasts. Ultimately, these fibroblasts are activated to become myofibroblasts, which secrete excessive amounts of extracellular matrix, resulting in increased tissue stiffness and loss of function of the alveolar tissue [20]. In addition, macrophages and lymphocytes are recruited to the site of injury and release pro-fibrotic mediators that further promote fibroblast activation [16]. In a feed-forward loop, the increased lung tissue stiffness further activates and stimulates fibroblasts, in a process known as mechano-stimulation, to drive self-sustaining and progressive lung fibrosis [21].
Mechanisms known to be involved in the pathogenesis and progression of fibrosing interstitial lung diseases. EMT: epithelial–mesenchymal transition; PDGF: platelet-derived growth factor; VEGF: vascular endothelial growth factor; bFGF: basic fibroblast growth factor; MVE: microvascular epithelium; FMT: fibroblast–myofibroblast transition; ECM: extracellular matrix. Tissue damage may occur at the alveolar epithelial and/or microvascular endothelial sites. Fibroblasts are recruited from resident fibroblasts, circulating fibrocytes, and epithelial cells and fibrocytes undergoing EMT. Growth factors are released by the damaged epithelium and endothelium, and leukocytes are recruited. Mononuclear cells and T-cells release pro-fibrotic mediators. Activated fibroblasts transition to myofibroblasts (FMT), which release excessive amounts of ECM. This results in increased tissue stiffness, which further stimulates fibroblast activation, leading to self-sustaining fibrosis.
Nintedanib
Nintedanib has been approved for the treatment of IPF in many countries, including Europe and the USA. The standard dosing in adults is 150 mg twice daily. Maximum plasma concentration at steady state after standard oral dosing in patients with IPF is ∼59–74 nmol·L−1 [22, 23].
Nintedanib is a small-molecule tyrosine kinase inhibitor that targets platelet-derived growth factor (PDGF) receptor-α and -β, fibroblast growth factor (FGF) receptor-1–3 and vascular endothelial growth factor (VEGF) receptor-1–3 [24]. Nintedanib binds competitively to the ATP-binding pocket of these receptors, thereby blocking intracellular signalling [24]. Nintedanib also inhibits the Src family kinase lymphocyte-specific tyrosine protein kinase (Lck), colony-stimulating factor (CSF)-1 receptor (CSF1R) and 20 other kinases with median inhibitory concentration (IC50) values <100 nM [24, 25]. The impact of enzymatic inhibition of most of these kinases on cellular functions has not yet been explored.
Mechanisms of action of nintedanib in pulmonary fibrosis
In vitro data have demonstrated that nintedanib inhibits a number of steps in the initiation and progression of lung fibrosis that might have relevance to the treatment of patients with progressive fibrosing ILDs.
Nintedanib exerts anti-inflammatory activity, attenuating the initiation and progression of fibrosis
On activation, T-cells release pro-inflammatory and pro-fibrotic mediators that are believed to initiate and enhance the progression of fibrosis in autoimmune diseases [26, 27]. Lck is activated after T-cell stimulation, and is required for T-cell proliferation and interleukin (IL)-2 production [28–31]. At an enzymatic level, nintedanib inhibits the activity of Lck with an IC50 of 16 nM [24]. In addition, nintedanib inhibits the release of mediators including IL-2, IL-4, IL-5, IL-10, IL-12p70, IL-13 and interferon-γ by human peripheral blood mononuclear cells or T-cells [32]. The potency of nintedanib is within the range of exposure observed in patients with IPF treated with the standard dose of 150 mg twice daily, suggesting that it is feasible that treatment with nintedanib would modulate these mediators during clinical use of the drug.
CCL18, a marker of M2 macrophage polarisation, has been associated with disease progression in patients with fibrosing ILDs, including IPF, SSc and RA [33–36]. It has been demonstrated that nintedanib prevents pro-fibrotic macrophage polarisation. When macrophages differentiated from human THP-1 cells exposed to a cocktail of IL-4/IL-13 or IL-4/IL-13 and IL-6 were treated with nintedanib at a concentration of 100 nM, the release of CCL18 was significantly reduced [37]. It is assumed that the inhibitory activity of nintedanib on CSF1R, which has been demonstrated at an enzymatic level with an IC50 of 5 nM [25], is at least partly responsible for this reduction. A recent study showed that nintedanib (0.01–1 μM) prevented the CSF1-induced phosphorylation of CSF1R, with a significant decrease in production of CCL2, adhesion of macrophages and polarisation of macrophages to alternative M2a macrophages [38]. Nintedanib has also been shown to inhibit alternative activation of monocytes. At a concentration of 100 nM, nintedanib significantly reduced M2 polarisation of human macrophages incubated with macrophage CSF, IL-4 and IL-13, as shown by changes in the mean fluorescence intensity of CD163 and CD206, while expression of M1 markers did not change [39].
Nintedanib inhibits fundamental processes of progressive lung fibrosis
Fibrocytes are a source of lung fibroblasts in fibrosing ILDs and are believed to promote fibrosis [40]. Nintedanib has been shown to attenuate the migration of fibrocytes, their differentiation to fibroblasts and the triggering of fibroblast proliferation by cultured fibrocytes [41]. Activated interstitial fibroblasts are the key effector cells in the pathogenesis of fibrosing ILDs. Nintedanib inhibits fibroblast proliferation induced by stimuli such as PDGF, FGF, VEGF [42, 43] and serum [44]. Nintedanib has also been shown to reduce the motility of lung fibroblasts from patients with IPF [45] and to inhibit the contraction of human lung fibroblasts in collagen gels stimulated with PDGF [46]. These effects are not limited to the lung: studies conducted on dermal fibroblasts from patients with SSc showed that nintedanib reduced their PDGF- and transforming growth factor (TGF)-β-induced proliferation and migration [47].
Nintedanib inhibits TGF-β-induced fibroblast to myofibroblast transformation [48] and the release of collagen from TGF-β-stimulated lung fibroblasts [37]. Upon TGF-β stimulation, pericytes, microvascular mural cells of mesenchymal origin, may achieve features of myofibroblasts and form fibroblastic foci. Nintedanib has been shown to inhibit deposition of fibronectin and collagen 1 by pericytes with myofibroblast features when they are cultured on decellularised lung matrix [49]. Furthermore, nintedanib reduces the enzymatic activity and secretion of matrix metalloproteinase (MMP)-2 and tissue inhibitor of MMP (TIMP)-2 by primary lung fibroblasts from patients with IPF [42], and increases MMP-8, MMP-9 and MMP-13 expression, and MMP-2 and MMP-9 activity, in pericytes activated with TGF-β1 [49]. Outside the lung, nintedanib reduced myofibroblast differentiation and collagen release from dermal fibroblasts from patients with SSc [47].
Nintedanib targets cells of the lung vasculature
The potential impact of attenuating vascular remodelling in patients with IPF and other fibrosing ILDs remains controversial [50, 51]. Nintedanib inhibits the proliferation of several vascular cell types in the lung, including endothelial cells, pericytes and smooth muscle cells [24, 52]. Nintedanib also inhibits the proliferation of human pulmonary artery vascular smooth muscle cells at basal conditions and on stimulation with PDGF [39]. Taken together, the experimental evidence supports nintedanib having effects on vascular cells; however, whether these effects add to its anti-fibrotic activity remains to be elucidated.
Studies of nintedanib in animal models of pulmonary fibrosis
Nintedanib has been explored in several animal models with features of fibrosing ILDs, such as IPF, SSc-ILD, RA-ILD, chronic hypersensitivity pneumonitis and silicosis. In these animal models, nintedanib was administered in a therapeutic manner, i.e. after the onset of substantial fibrosis. Nintedanib demonstrated anti-fibrotic activity irrespective of the trigger that caused the fibrotic lung pathology, supporting the hypothesis that nintedanib inhibits fundamental processes in the pathogenesis of fibrosis. In addition to anti-fibrotic efficacy, nintedanib demonstrated anti-inflammatory activity and effects on the vascular compartment. In some animal models, nintedanib also showed effects on aspects of the disease other than fibrosing ILD, such as on heart fibrosis in a model of SSc-ILD, and on airway hyperreactivity and inflammation in a model showing features of chronic hypersensitivity pneumonitis. An overview of the different models is given in table 1.
Overview of nonclinical exploration of nintedanib in animal models of lung fibrosis
Chemical trigger: bleomycin-induced lung fibrosis
In rodents, intratracheal administration of bleomycin elicits severe epithelial damage, pulmonary inflammation, a massive fibrotic response in the lung and irregular vascularisation. In three animal models of bleomycin-induced lung fibrosis, nintedanib demonstrated anti-fibrotic, anti-inflammatory and vascular remodelling activity [43, 48, 53]. Anti-fibrotic activity was demonstrated through significant reductions in lung tissue density determined by micro computed tomography (CT), Ashcroft score, protein expression of TIMP-1, mRNA expression of TGF-β1 and pro-collagen 1, and the collagen content of lung tissue, as well as by an improvement in lung function, as determined by static compliance calculated from pressure–volume loops. In addition, nintedanib showed anti-inflammatory activity, including attenuation of the accumulation of lymphocytes in bronchoalveolar lavage fluid (BALF) and a reduction in IL-1β and chemokine CXCL1/KC. In a mouse model of bleomycin-induced lung fibrosis, nintedanib normalised the distorted vascular architecture and inhibited proliferation of alveolar macrophages and alveolar epithelial type II cells [53].
Environmental trigger: silica crystal-induced lung fibrosis
Intratracheal application of silica crystals in mice results in ongoing lung inflammation and a progressive fibrotic response resembling features of silicosis. In this model, nintedanib reduced lung inflammation and granuloma formation and reduced neutrophil and lymphocyte numbers in the BALF, and IL-1β, CXCL1/KC and IL-6 levels in lung tissue homogenates. Anti-fibrotic activity of nintedanib was demonstrated by a reduction in fibrosis in lung histology and reduced collagen levels in lung tissue [48].
Allergic trigger: ovalbumin-induced fibrotic lung remodelling
In mice, chronic allergic stimulation of the lung with ovalbumin results in features such as airway hyperreactivity, lung inflammation and goblet cell hyperplasia, and ultimately also in airway remodelling with fibrosis, resembling not only asthma, but also aspects of chronic hypersensitivity pneumonitis. In this model, treatment with nintedanib led to a significant reduction in airway hyperreactivity and a reduction in the hydroxyproline content of the lungs, indicating anti-fibrotic activity [54].
Immunological trigger: zymosan-induced arthritis and lung fibrosis in SKG mice
In SKG mice (which contain a Zap70 mutation resulting in autoreactive T-cells), administration of zymosan induces an arthritis pathology. The arthritis score increases for 6 weeks after zymosan administration, remains high up to week 10 and then slowly decreases. At least 20% of SKG mice treated with zymosan develop lung fibrosis, reflected in an increase in lung collagen (hydroxyproline) content at week 10, which increases further until week 16. Treatment of SKG mice with nintedanib in weeks 5–11 after zymosan administration has been shown to attenuate arthritis pathology with no effect on lung fibrosis, while nintedanib treatment in weeks 10–16 led to a reduction in collagen deposition in the lungs. Nintedanib seemed to trigger a slight but selective inflammation in the lung; the impact of this with respect to the fibrosis is unknown [55].
Vascular trigger: vascular lesions, skin, lung and heart fibrosis in Fra-2+/– mice
Transgenic overexpression of fos-related antigen-2 (Fra-2) in mice results in development of microvascular disease and pulmonary hypertension. Fra-2 transgenic mice develop progressive fulminant skin fibrosis with dermal thickening, increased hydroxyproline content and an increase in myofibroblasts. Fibrosis in these animals also affects the lung. In this model, treatment with nintedanib from age 9 to 16 weeks effectively attenuated fibrosis in the skin and lung, with a near normalisation of dermal thickness, a reduction in the hydroxyproline content of the skin and lungs, and reduced myofibroblast counts in both organs. Furthermore, nintedanib prominently inhibited pulmonary arterial hypertension-like changes and reduced the proliferation of pulmonary vascular smooth muscle cells, the thickening of vascular walls of pulmonary arteries, and the number of occluded vessels. In the skin, nintedanib reduced apoptosis in dermal microvascular endothelial cells, increased the number of endothelial cells (a marker of vascularisation) and prevented the increase in pro-fibrotic M2 polarised macrophages [39].
Additional animal models
Experiments in other animal models have demonstrated the activity of nintedanib on fundamental processes of fibrosis. Nintedanib has shown anti-fibrotic activity in mouse models of skin fibrosis [47], radiation-induced lung fibrosis [56], polyhexamethylene guanidine phosphate-induced lung fibrosis [57], renal fibrosis [58], liver fibrosis [59] and muscle fibrosis [60].
Clinical investigation of nintedanib in patients with progressive fibrosing ILDs
Based on the aforementioned evidence from nonclinical studies that nintedanib inhibits fundamental processes of progressive fibrosis, irrespective of the trigger, and the established efficacy of nintedanib in slowing disease progression in patients with IPF [61, 62], nintedanib has been investigated as a potential treatment for other fibrosing ILDs with a progressive phenotype. The SENSCIS trial (ClinicalTrials.gov identifier NCT02597933) investigated the efficacy and safety of nintedanib versus placebo in patients with SSc-ILD, an extent of fibrotic disease ≥10% on high-resolution CT (HRCT) and onset of SSc 7 years before screening [63]. Nintedanib significantly reduced the annual rate of decline in forced vital capacity (FVC) assessed over 52 weeks (primary end-point) (−52.4 versus −93.3 mL·year−1). The rate of FVC decline was lower in patients with SSc-ILD in the SENSCIS trial than in patients with IPF in the INPULSIS trials [62], but the relative reduction in the rate of FVC decline observed with nintedanib versus placebo was similar (44% and 49%, respectively). The INBUILD trial (ClinicalTrials.gov identifier NCT02999178) assessed the efficacy and safety of nintedanib 150 mg twice daily versus placebo in patients with a physician-diagnosed ILD other than IPF, who have features of diffuse fibrosing lung disease of >10% extent on HRCT [64]. To be eligible to enter the INBUILD trial, patients had to meet criteria for disease progression in the 2 years prior to screening, based on decline in FVC, worsening of respiratory symptoms or an increased extent of fibrotic changes on HRCT. As in the SENSCIS trial, the primary end-point is the annual rate of decline in FVC (mL·year−1) assessed over 52 weeks. There are two co-primary analysis populations: all patients and patients with a usual interstitial pneumonia (UIP)-like fibrotic pattern on HRCT; patients with ILD and a UIP-like pattern on HRCT have been shown in a number of studies to be at greater risk of FVC decline than patients with other patterns on HRCT [5, 10, 65]. The results of the INBUILD trial will be reported in 2019.
Conclusions
Fibrosing ILDs with a progressive phenotype show commonalities both in clinical behaviour and in the pathogenic mechanisms that drive their progression. Nintedanib is an approved treatment for IPF and has recently been shown to reduce the rate of progression of ILD in patients with SSc-ILD. Data from in vitro and in vivo studies have shown that nintedanib inhibits a number of steps in the initiation and progression of lung fibrosis. Furthermore, nintedanib has demonstrated anti-fibrotic effects in several animal models with features of fibrosing ILDs, irrespective of the trigger that caused the fibrotic lung pathology. A large clinical trial of nintedanib in patients with progressive fibrosing ILDs other than IPF will shortly report results.
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Acknowledgments
Writing assistance, supported financially by Boehringer Ingelheim, was provided by Wendy Morris (FleishmanHillard Fishburn, London, UK) during the preparation of this article. The authors were fully responsible for all content and editorial decisions, were involved at all stages of manuscript development, and have approved the final version.
Footnotes
Conflict of interest: L. Wollin is an employee of Boehringer Ingelheim Pharma GmbH & Co. KG.
Conflict of interest: J.H.W. Distler has nothing to disclose.
Conflict of interest: E.F. Redente has nothing to disclose.
Conflict of interest: D.W.H. Riches has nothing to disclose.
Conflict of interest: S. Stowasser is an employee of Boehringer Ingelheim International GmbH.
Conflict of interest: R. Schlenker-Herceg is an employee of Boehringer Ingelheim Pharmaceuticals, Inc.
Conflict of interest: T.M. Maher has, via his institution, received industry-academic funding from GlaxoSmithKline R&D and UCB; has received consultancy or speakers fees from Apellis, AstraZeneca, Bayer, Biogen Idec, Boehringer Ingelheim, Galapagos, GlaxoSmithKline R&D, Indalo, Pliant, ProMetic, Roche, Samumed and UCB; and has received consultancy fees from Galecto.
Conflict of interest: M. Kolb reports grants and personal fees for consultancy and lecturing from Roche and Boehringer Ingelheim, grants and personal fees for consultancy from GSK, Gilead and Prometic, grants from Actelion, Respivert, Alkermes and Pharmaxis, personal fees for consultancy from Genoa, Indalo and Third Pole, outside the submitted work.
- Received January 23, 2019.
- Accepted June 20, 2019.
- Copyright ©ERS 2019
This version is distributed under the terms of the Creative Commons Attribution Non-Commercial Licence 4.0.
References
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- Abstract
- Abstract
- Introduction
- Pathogenesis of fibrosing ILDs with progressive phenotype
- Nintedanib
- Mechanisms of action of nintedanib in pulmonary fibrosis
- Studies of nintedanib in animal models of pulmonary fibrosis
- Clinical investigation of nintedanib in patients with progressive fibrosing ILDs
- Conclusions
- Shareable PDF
- Acknowledgments
- Footnotes
- References
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