Abstract
Photobiomodulation (PB) demonstrates efficacy in lung diseases, reducing inflammatory parameters. Corticosteroids (Fluticasone - FT), are used in the treatment asthma, but it has many side effects. The objective was to evaluate the effect of PB on asthmatic animals submitted or no to the FT treatment. Balb/C mice divided into groups: controls, OVA, OVA+PB, OVA+FT and OVA+PB+FT. Induction: sensitization with Ovalbumin (OVA), and orotracheal challenge (3 days/week for 5 weeks), PB (diode laser - 660nm, 30mW and 3J) and FT (100 μg/kg - intranasal). At day 57, functional and structural parameters were analyzed. The data were submitted to the One-way ANOVA test followed by the Newman-Keuls test (5%-p<0.05). We observed a reduction in the total number of cells in BAL (p<0.001), macrophages (p<0.05), with best result in OVA+PB (p<0.001), lymphocytes (p<0.05), with greater reduction in the groups associated to PB (p<0.001), neutrophils and eosinophils (p<0.001). Reduction of IL-4, IL-5, IL1-β, TNF-α (p<0.001) and IL-13 (p<0.01) where the PB treated groups showed better results (p<0.001), increased the level of the IL-10 (p<0.01) presenting in the groups treated with PB (p<0.001). There was reduction of collagen fibers and mucus in the airways (p<0.001) and the tracheal responsiveness to methacholine (MCh) (p<0.05) and OVA+PB (p<0.01). We conclude that the therapies are efficient in reducing the inflammatory parameters. However, the combination of therapies maybe considered a promising tool for the treatment of patients with asthma. Perhaps, low doses of corticosteroids associated with PB have better results for the treatment of this disease.
Footnotes
Cite this article as: European Respiratory Journal 2018 52: Suppl. 62, PA1057.
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