Abstract
Acute Lung Injury(ALI) is a clinical manifestation of respiratory failure caused by a response of the lung against an injury. The damage to the alveolar barrier is a critical event and it could be repaired by alveolar type IIcells(ATII) or by mesenchymal stem cells(MSC)trasplantation.
Objective: Compare the involved pathways in the ALI resolution after ATII and MSC cells administration.
Sprague-Dawley rats(∼300g) were sedated and lung injury was induced by intratracheal instillation of HCl(0.1mol/L),followed 2h later by instillation of LPS of E.coli(30μg/g body weight).Control rats were treated with saline.9h after the second instillation ATII cells or MSC (2.5x106cells)were transplanted intratracheally.Data are expressed as mean±SEM. Statistical analysis was performed with OneWay-ANOVA and NewmanKeuls post-hoc.Statistical significance p<0.05was considered.
The instillation of HCl+LPS caused an increase in lung weight,TNFα, IL1ß and IL6. Transplantation of both cell populations reduced significantly all of them. ATII and MSC cell therapy presented marked differences.ATII cells had a huge antinflammatory activity, reducing to level controls IL4 and IL13. MSC transplantation did not produce any change in IL4 or IL13 compared to the HCl+LPSgroup. On the other hand,MSC transplantation induces a reduction in GM-CSF indicating a less number of monocytes recruited to the lung. ATII cells transplantation produces a better recovering of the lung injury evaluated by histological analysis.
Conclusion: Both populations of cells are effective in lung injury recovery. The study of the specific pathways involved in the recovery of lung injury allows us to improve a better therapy for ALI/ARDS.
- Copyright ©the authors 2016