Abstract
Asthma is defined as a chronic lung disease of the airways associated with bronchial hyperresponsiveness with significant airflow limitation. Experimental models have been proposed to advance knowledge about the disease, as well as the discovery of new therapies. The Low Level Laser Therapy (LLLT) is a relatively new and effective, low cost, no side effects and possible use in the treatment of lung diseases. In this sense, aim of this study was evaluate the effect of LLLT 3J on chronic pulmonary inflammation in experimental model of asthma induced by HDM. BALB/c mice were divided four groups: control, HDM, LLLT 3J, HDM+LLLT 3J. Application of LLLT was 1 hour after the administration of HDM, with a wavelength of 660nm, power of 100m. Experimental model the animals were anesthetized with ketamine and xylazine and each animal received intra-tracheal administration of 100ug of HDM on days 0, 14, followed by three weekly administrations to day 42. On day 43, the animals were sacrificed and studied functional and structurally. Results indicate that LLLT reduced pulmonary inflammation by reducing the number of total cells in BAL (p<0.05), as well as reduction of eosinophils (p <0.05). LLLT incresead the BAL level of IL-10 (p <0.05), evaluated by ELISA. LLLT reduced the level of the bronchoconstriction index (p <0.05). LY6G and CD3 levels were significantly reduced in the group associated with LLLT (p <0.05). In contrast, levels of CD11b is present elevated (p <0.05) evaluated bu lung homogenate. Conclusion, the LLLT inhibited the pulmonary inflammation and the eosinophilic infiltration, as well as decreased the bronchoconstriction index and significantly increased levels of IL-10 in BAL.
- Copyright ©the authors 2016