Skip to main content

Main menu

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • For authors
    • Instructions for authors
    • Submit a manuscript
    • Author FAQs
    • Open access
    • COVID-19 submission information
  • Alerts
  • Podcasts
  • Subscriptions
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

User menu

  • Log in
  • Subscribe
  • Contact Us
  • My Cart

Search

  • Advanced search
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

Login

European Respiratory Society

Advanced Search

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • For authors
    • Instructions for authors
    • Submit a manuscript
    • Author FAQs
    • Open access
    • COVID-19 submission information
  • Alerts
  • Podcasts
  • Subscriptions

The role of amiloride sensitive epithelial sodium channel (ENaC) in cell migration of mesothelioma cells is dependent on the cell type and the extracellular matrix

Rajesh Jagirdar, Paschalis Adam Molyvdas, Konstantinos Gourgoulianis, Chrissi Hatzoglou, Sotirios Zarogiannis
European Respiratory Journal 2016 48: PA5072; DOI: 10.1183/13993003.congress-2016.PA5072
Rajesh Jagirdar
1Department of Physiology, Faculty of Medicine, University of Thessaly, Larissa, Greece
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Paschalis Adam Molyvdas
1Department of Physiology, Faculty of Medicine, University of Thessaly, Larissa, Greece
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Konstantinos Gourgoulianis
2Department of Respiratory Medicine, Faculty of Medicine, University of Thessaly, Larissa, Greece
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chrissi Hatzoglou
1Department of Physiology, Faculty of Medicine, University of Thessaly, Larissa, Greece
2Department of Respiratory Medicine, Faculty of Medicine, University of Thessaly, Larissa, Greece
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sotirios Zarogiannis
1Department of Physiology, Faculty of Medicine, University of Thessaly, Larissa, Greece
2Department of Respiratory Medicine, Faculty of Medicine, University of Thessaly, Larissa, Greece
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
Loading

Abstract

Background: Malignant pleural mesothelioma (MPM) is an aggressive malignancy. In MPM cells the amiloride sensitive sodium channel (ENaC) is expressed, however its role in cell migration is unknown.

Aim: Our aim was to investigate the role of ENaC inhibition on MPM cell migration during wound healing.

Materials and methods: The human cell lines MeT-5A (benign mesothelial cells), M14K (epithelioid MPM), MSTO (biphasic MPM) and ZL34 (sarcomatoid MPM) were used in this study. Cell migration was tested at 4 hrs on fibronectin (FN) and at 8 hrs on homologous cell derived extracellular matrix (ECM) coated plates. ENaC inhibition was done by amiloride (AM, 10-5M). Migration index (MI) was used as a measure of cell migration.

Results: The migration of MeT-5A (MI=0.06±0.01; p<0.001) and M14K (MI=0.09±0.01; p<0.005) cells were significantly lower than both MSTO (MI=0.22±0.01) and ZL34 (MI=0.59±0.03) cells on FN coated plates. Additionally, MSTO cell migration was significantly lower than ZL34 (p<0.001). On ECM coated plates, MeT-5A (MI=0.18±0.01) was significantly lower than MSTO (MI=0.33±0.01; p<0.01) and ZL34 (MI=0.90±0.02; p<0.001), while migration of M14K (MI=0.23±0.01) was significantly lower than MSTO (p<0.01) and ZL34 (p<0.001). Inhibition of ENaC with AM showed significant decrease of the cell migration in MeT-5A (p<0.05) and ZL34 (p<0.05) on FN coated plates. On ECM, the inhibitory effect of AM on cell migration was shown on MSTO (p<0.05) and ZL34 (p<0.05) cells.

Conclusion: ENaC inhibition differentially decreases the cell migration of normal mesothelial and MPM cells in a histological and substartum dependent manner.

  • Mesothelioma
  • Cell biology
  • Pleura
  • Copyright ©the authors 2016
Previous
Back to top
Vol 48 Issue suppl 60 Table of Contents
  • Table of Contents
  • Index by author
Email

Thank you for your interest in spreading the word on European Respiratory Society .

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
The role of amiloride sensitive epithelial sodium channel (ENaC) in cell migration of mesothelioma cells is dependent on the cell type and the extracellular matrix
(Your Name) has sent you a message from European Respiratory Society
(Your Name) thought you would like to see the European Respiratory Society web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Alerts
Sign In to Email Alerts with your Email Address
Citation Tools
The role of amiloride sensitive epithelial sodium channel (ENaC) in cell migration of mesothelioma cells is dependent on the cell type and the extracellular matrix
Rajesh Jagirdar, Paschalis Adam Molyvdas, Konstantinos Gourgoulianis, Chrissi Hatzoglou, Sotirios Zarogiannis
European Respiratory Journal Sep 2016, 48 (suppl 60) PA5072; DOI: 10.1183/13993003.congress-2016.PA5072

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
The role of amiloride sensitive epithelial sodium channel (ENaC) in cell migration of mesothelioma cells is dependent on the cell type and the extracellular matrix
Rajesh Jagirdar, Paschalis Adam Molyvdas, Konstantinos Gourgoulianis, Chrissi Hatzoglou, Sotirios Zarogiannis
European Respiratory Journal Sep 2016, 48 (suppl 60) PA5072; DOI: 10.1183/13993003.congress-2016.PA5072
del.icio.us logo Digg logo Reddit logo Technorati logo Twitter logo CiteULike logo Connotea logo Facebook logo Google logo Mendeley logo

Jump To

  • Article
  • Info & Metrics
  • Tweet Widget
  • Facebook Like
  • Google Plus One

More in this TOC Section

  • Comparison between mesothelin and fibulin-3 detection in pleural malignant mesothelioma (MPM) patients
  • A comparative study of conventional cytology and cell block method in the diagnosis of pleural effusion
  • An examination of the factors for successful pleurodesis against malignant pleural effusion (MPE)
Show more 11.2 Pleural and Mediastinal Malignancies

Related Articles

Navigate

  • Home
  • Current issue
  • Archive

About the ERJ

  • Journal information
  • Editorial board
  • Reviewers
  • CME
  • Press
  • Permissions and reprints
  • Advertising

The European Respiratory Society

  • Society home
  • myERS
  • Privacy policy
  • Accessibility

ERS publications

  • European Respiratory Journal
  • ERJ Open Research
  • European Respiratory Review
  • Breathe
  • ERS books online
  • ERS Bookshop

Help

  • Feedback

For authors

  • Instructions for authors
  • Submit a manuscript
  • ERS author centre

For readers

  • Alerts
  • Subjects
  • Podcasts
  • RSS

Subscriptions

  • Accessing the ERS publications

Contact us

European Respiratory Society
442 Glossop Road
Sheffield S10 2PX
United Kingdom
Tel: +44 114 2672860
Email: journals@ersnet.org

ISSN

Print ISSN:  0903-1936
Online ISSN: 1399-3003

Copyright © 2021 by the European Respiratory Society