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Effect of high, medium, and low molecular weight hyaluronan on inflammation and oxidative stress in an in vitro model of human nasal epithelial cells

Giusy D. Albano, Anna Bonanno, Luca Cavalieri, Eleonora Ingrassia, Caterina Di Sano, Liboria Siena, Loredana Riccobono, Rosalia Gagliardo, Mirella Profita
European Respiratory Journal 2016 48: PA5064; DOI: 10.1183/13993003.congress-2016.PA5064
Giusy D. Albano
1IBIM, CNR, Palermo, Israel
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Anna Bonanno
1IBIM, CNR, Palermo, Israel
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Luca Cavalieri
2Farmaceutici SpA, Chiesi, Parma, Italy
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Eleonora Ingrassia
2Farmaceutici SpA, Chiesi, Parma, Italy
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Caterina Di Sano
1IBIM, CNR, Palermo, Israel
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Liboria Siena
1IBIM, CNR, Palermo, Israel
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Loredana Riccobono
1IBIM, CNR, Palermo, Israel
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Rosalia Gagliardo
1IBIM, CNR, Palermo, Israel
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Mirella Profita
1IBIM, CNR, Palermo, Israel
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Abstract

We investigated the pro or anti-inflammatory Hyaluronan (HA) activity at different molecular weights (MW) in an in vitro model of nasal inflammation IL-17A mediated. We evaluated the cytoplasmic ERK1/2 and IkBα phosphorylation,nuclear NF-kB signal pathway activation, ROS production, IL-8 and NOX-4 protein and mRNA levels, in nasal epithelial cells RPMI 2650 stimulated with recombinant human (rhIL-17A). The cells were treated with rhIL-17A in the presence or absence of HMW-HA (High-MW-HA; ∼1600 kDa), MMW-HA (Medium-MW-HA;∼900 kDa), LMW-HA (Low-MW-HA;500 kDa), and U0126 (specific inhibitor of MEK1and MEK2 MAP kinase kinase; MAPKK). We showed that rhIL-17A increased the ERK1/2, IkBα phosphorylation, NF-kB signal pathway activation, ROS production, IL-8 and NOX-4 proteins and mRNA levels. The addiction of HMW-HA or U0126, rather than MMW-HA or LMW-HA, showed a statistical significant down-regulatory effect on ERK1/2and IkBα phosphorylation, NF-kB signal pathway activation,ROS production, IL-8 and NOX-4 proteins in nasal epithelial cells stimulated with rhIL-17A. IL-17A might generate oxidative stress and inflammation via the activation ERK1/2/NFkB pathway in nasal epithelial cells. The HMW-HA might represent a coadjuvant of the classic anti-inflammatory/anti-oxidative treatment of nasal epithelial cells during IL-17A nasal inflammation.

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Effect of high, medium, and low molecular weight hyaluronan on inflammation and oxidative stress in an in vitro model of human nasal epithelial cells
Giusy D. Albano, Anna Bonanno, Luca Cavalieri, Eleonora Ingrassia, Caterina Di Sano, Liboria Siena, Loredana Riccobono, Rosalia Gagliardo, Mirella Profita
European Respiratory Journal Sep 2016, 48 (suppl 60) PA5064; DOI: 10.1183/13993003.congress-2016.PA5064

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Effect of high, medium, and low molecular weight hyaluronan on inflammation and oxidative stress in an in vitro model of human nasal epithelial cells
Giusy D. Albano, Anna Bonanno, Luca Cavalieri, Eleonora Ingrassia, Caterina Di Sano, Liboria Siena, Loredana Riccobono, Rosalia Gagliardo, Mirella Profita
European Respiratory Journal Sep 2016, 48 (suppl 60) PA5064; DOI: 10.1183/13993003.congress-2016.PA5064
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