Skip to main content

Main menu

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • ERS Guidelines
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • Open access
    • COVID-19 submission information
    • Peer reviewer login
  • Alerts
  • Subscriptions
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

User menu

  • Log in
  • Subscribe
  • Contact Us
  • My Cart

Search

  • Advanced search
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

Login

European Respiratory Society

Advanced Search

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • ERS Guidelines
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • Open access
    • COVID-19 submission information
    • Peer reviewer login
  • Alerts
  • Subscriptions

Pharmacological characterization of the anti-oxidant activity of N-acetylcysteine in an ex vivo model of COPD exacerbation

Paola Rogliani, Josuel Ora, Luigino Calzetta, Gabriella Lucà, Maria Gabriella Matera, Mario Cazzola
European Respiratory Journal 2016 48: PA5062; DOI: 10.1183/13993003.congress-2016.PA5062
Paola Rogliani
1Systems Medicine, University of Rome Tor Vergata, Rome, Italy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Josuel Ora
1Systems Medicine, University of Rome Tor Vergata, Rome, Italy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Luigino Calzetta
1Systems Medicine, University of Rome Tor Vergata, Rome, Italy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gabriella Lucà
1Systems Medicine, University of Rome Tor Vergata, Rome, Italy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Maria Gabriella Matera
2Experimental Medicine, Second University of Naples, Naples, Italy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mario Cazzola
1Systems Medicine, University of Rome Tor Vergata, Rome, Italy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
Loading

Abstract

Background The oxidant/antioxidant imbalance is significantly pronounced in patients with COPD exacerbation. N-acetylcysteine (NAC) seems to have an influence in reducing COPD exacerbations via modulating the oxidative stress, but there are discordant findings on the actual anti-oxidant activity of NAC.

Aim We aimed to pharmacologically characterize the anti-oxidant effect of NAC in an ex vivo model of COPD exacerbation.

Methods The influence of NAC was assessed on the contractile response and anti-oxidant profile in human isolated bronchi incubated overnight with Krebs-Henseleit buffer solution (control) or lipopolysaccharide (LPS, 100 ng/ml).

Results LPS desensitized isolated airways against the transmural stimulation (-55%±13%, P<0.05 vs. control). Concentrations of NAC ≥10μM restored the physiological contractile response in LPS stimulated bronchi (Emax 99±19% vs. control, P<0.05 vs. LPS), whereas lower concentrations (<10μM) did not modulate this effect. Concentrations of NAC ≥1μM reduced the pro-oxidant response (peroxidase activity, hydrogen peroxide, malondialdehyde, nitric oxide: overall -31±2%; P<0.05 vs. LPS), and improved the anti-oxidant response (total anti-oxidant capacity, glutathione, superoxide dismutase: overall +97±8%; P<0.05 vs. LPS) induced by LPS. Lower concentrations of NAC (<1μM) did not modulate the bronchial oxidative imbalance (P>0.05 vs. LPS).

Conclusions The results of this study demonstrated that concentrations of NAC ≥10μM are required to normalize the dysfunctional contractility of human bronchi and concentrations of NAC ≥1μM improve the oxidative imbalance in an ex vivo model of COPD exacerbation.

  • Airway smooth muscle
  • COPD - exacerbations
  • Pharmacology
  • Copyright ©the authors 2016
Previous
Back to top
Vol 48 Issue suppl 60 Table of Contents
  • Table of Contents
  • Index by author
Email

Thank you for your interest in spreading the word on European Respiratory Society .

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Pharmacological characterization of the anti-oxidant activity of N-acetylcysteine in an ex vivo model of COPD exacerbation
(Your Name) has sent you a message from European Respiratory Society
(Your Name) thought you would like to see the European Respiratory Society web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Pharmacological characterization of the anti-oxidant activity of N-acetylcysteine in an ex vivo model of COPD exacerbation
Paola Rogliani, Josuel Ora, Luigino Calzetta, Gabriella Lucà, Maria Gabriella Matera, Mario Cazzola
European Respiratory Journal Sep 2016, 48 (suppl 60) PA5062; DOI: 10.1183/13993003.congress-2016.PA5062

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Pharmacological characterization of the anti-oxidant activity of N-acetylcysteine in an ex vivo model of COPD exacerbation
Paola Rogliani, Josuel Ora, Luigino Calzetta, Gabriella Lucà, Maria Gabriella Matera, Mario Cazzola
European Respiratory Journal Sep 2016, 48 (suppl 60) PA5062; DOI: 10.1183/13993003.congress-2016.PA5062
del.icio.us logo Digg logo Reddit logo Technorati logo Twitter logo CiteULike logo Connotea logo Facebook logo Google logo Mendeley logo

Jump To

  • Article
  • Info & Metrics
  • Tweet Widget
  • Facebook Like
  • Google Plus One

More in this TOC Section

  • TRPM3: A regulator of airway sensory nerves and respiratory reflexes
  • Anti-inflammatory activity of the bradykinin B1R antagonist in a model of LPS-induced lung inflammation in cynomolgus monkey
  • Prostacyclin IP receptor activation blocks hyperosmolar-induced bronchoconstriction in isolated human small airways
Show more 5.1 Airway Pharmacology and Treatment

Related Articles

Navigate

  • Home
  • Current issue
  • Archive

About the ERJ

  • Journal information
  • Editorial board
  • Press
  • Permissions and reprints
  • Advertising

The European Respiratory Society

  • Society home
  • myERS
  • Privacy policy
  • Accessibility

ERS publications

  • European Respiratory Journal
  • ERJ Open Research
  • European Respiratory Review
  • Breathe
  • ERS books online
  • ERS Bookshop

Help

  • Feedback

For authors

  • Instructions for authors
  • Publication ethics and malpractice
  • Submit a manuscript

For readers

  • Alerts
  • Subjects
  • Podcasts
  • RSS

Subscriptions

  • Accessing the ERS publications

Contact us

European Respiratory Society
442 Glossop Road
Sheffield S10 2PX
United Kingdom
Tel: +44 114 2672860
Email: journals@ersnet.org

ISSN

Print ISSN:  0903-1936
Online ISSN: 1399-3003

Copyright © 2023 by the European Respiratory Society