Skip to main content

Main menu

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • Open access
    • COVID-19 submission information
    • Peer reviewer login
  • Alerts
  • Podcasts
  • Subscriptions
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

User menu

  • Log in
  • Subscribe
  • Contact Us
  • My Cart

Search

  • Advanced search
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

Login

European Respiratory Society

Advanced Search

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • Open access
    • COVID-19 submission information
    • Peer reviewer login
  • Alerts
  • Podcasts
  • Subscriptions

Effects of phosphoinositide-3-kinase inhibitors on steroid-insensitive airway inflammation in poly(I:C) and allergen challenged mice

Genki Kimura, Yuki Nishimoto, Keitaro Ueda, Takahiro Nakaoki, Takashi Masuko, Peter J. Barnes, Kazuhiro Ito, Yasuo Kizawa
European Respiratory Journal 2016 48: PA4896; DOI: 10.1183/13993003.congress-2016.PA4896
Genki Kimura
1Physiology and anatomy, Nihon University School of Pharmacy, Funabashi, ChibaJapan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yuki Nishimoto
1Physiology and anatomy, Nihon University School of Pharmacy, Funabashi, ChibaJapan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Keitaro Ueda
1Physiology and anatomy, Nihon University School of Pharmacy, Funabashi, ChibaJapan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Takahiro Nakaoki
1Physiology and anatomy, Nihon University School of Pharmacy, Funabashi, ChibaJapan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Takashi Masuko
1Physiology and anatomy, Nihon University School of Pharmacy, Funabashi, ChibaJapan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Peter J. Barnes
2NHLI, Imperial College, London, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kazuhiro Ito
2NHLI, Imperial College, London, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yasuo Kizawa
1Physiology and anatomy, Nihon University School of Pharmacy, Funabashi, ChibaJapan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
Loading

Abstract

Viral infections induce exacerbations in asthmatic patients with increased airway inflammation that is corticosteroid-insensitive. The aim of this study was to evaluate the effects of phosphoinositide-3-kinase (PI3K) inhibitors on airway inflammation in induced by the viral mimic poly(I:C) in combination with ovalbumin in sensitized mice. A/J male mice were sensitized and challenged by OVA. Then poly(I:C) was given intranasally for 3 days, and AS604850 (AS; PI3Kγ inhibitor), IC87114 (IC; PI3Kδ inhibitor) and/or fluticasone propionate (FP, a corticosteroid) were administered intranasally 2h before each poly(I:C) exposure. Bronchoalveolar lavage fluid (BALF) was collected at 24h after the last poly(I:C) exposure. Macrophages, neutrophils and eosinophils in BALF were quantified by FACS analysis. The levels of CXCL1 and TNF-α in BALF were determined using a ELISA kit. Poly(I:C) exposure caused a significant increase in BALF macrophages, neutrophils, eosinophils, CXCL1 and TNF-α, which was not inhibited by FP (0.05 mg/ml). Neither AS (4.0 mg/ml) nor IC (4.0 mg/ml) alone inhibited airway inflammation, while the combination of IC and FP demonstrated greater inhibition on the inflammation in poly(I:C) and OVA-exposed mice. In contrast, the combination of AS and FP did not show anti-inflammatory activity. These data suggest that PI3Kδ inhibitor combined with a corticosteroid offers potential treatment for corticosteroid insensitive airway inflammation during viral infections.

  • Animal models
  • Asthma - mechanism
  • Inflammation
  • Copyright ©the authors 2016
Previous
Back to top
Vol 48 Issue suppl 60 Table of Contents
  • Table of Contents
  • Index by author
Email

Thank you for your interest in spreading the word on European Respiratory Society .

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Effects of phosphoinositide-3-kinase inhibitors on steroid-insensitive airway inflammation in poly(I:C) and allergen challenged mice
(Your Name) has sent you a message from European Respiratory Society
(Your Name) thought you would like to see the European Respiratory Society web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Effects of phosphoinositide-3-kinase inhibitors on steroid-insensitive airway inflammation in poly(I:C) and allergen challenged mice
Genki Kimura, Yuki Nishimoto, Keitaro Ueda, Takahiro Nakaoki, Takashi Masuko, Peter J. Barnes, Kazuhiro Ito, Yasuo Kizawa
European Respiratory Journal Sep 2016, 48 (suppl 60) PA4896; DOI: 10.1183/13993003.congress-2016.PA4896

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Effects of phosphoinositide-3-kinase inhibitors on steroid-insensitive airway inflammation in poly(I:C) and allergen challenged mice
Genki Kimura, Yuki Nishimoto, Keitaro Ueda, Takahiro Nakaoki, Takashi Masuko, Peter J. Barnes, Kazuhiro Ito, Yasuo Kizawa
European Respiratory Journal Sep 2016, 48 (suppl 60) PA4896; DOI: 10.1183/13993003.congress-2016.PA4896
del.icio.us logo Digg logo Reddit logo Technorati logo Twitter logo CiteULike logo Connotea logo Facebook logo Google logo Mendeley logo

Jump To

  • Article
  • Info & Metrics
  • Tweet Widget
  • Facebook Like
  • Google Plus One

More in this TOC Section

  • TRPM3: A regulator of airway sensory nerves and respiratory reflexes
  • Anti-inflammatory activity of the bradykinin B1R antagonist in a model of LPS-induced lung inflammation in cynomolgus monkey
  • Prostacyclin IP receptor activation blocks hyperosmolar-induced bronchoconstriction in isolated human small airways
Show more 5.1 Airway Pharmacology and Treatment

Related Articles

Navigate

  • Home
  • Current issue
  • Archive

About the ERJ

  • Journal information
  • Editorial board
  • Reviewers
  • Press
  • Permissions and reprints
  • Advertising

The European Respiratory Society

  • Society home
  • myERS
  • Privacy policy
  • Accessibility

ERS publications

  • European Respiratory Journal
  • ERJ Open Research
  • European Respiratory Review
  • Breathe
  • ERS books online
  • ERS Bookshop

Help

  • Feedback

For authors

  • Instructions for authors
  • Publication ethics and malpractice
  • Submit a manuscript

For readers

  • Alerts
  • Subjects
  • Podcasts
  • RSS

Subscriptions

  • Accessing the ERS publications

Contact us

European Respiratory Society
442 Glossop Road
Sheffield S10 2PX
United Kingdom
Tel: +44 114 2672860
Email: journals@ersnet.org

ISSN

Print ISSN:  0903-1936
Online ISSN: 1399-3003

Copyright © 2022 by the European Respiratory Society