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Sputum levels of miR-145 and miR-338 in COPD, asthma and ACOS subjects

Donato Lacedonia, Grazia Pia Palladino, Maria Pia Foschino Barbaro, Cristina Colanardi, Piera Soccio, Incoronata Caccavo, Giovanna E. Carpagnano
European Respiratory Journal 2016 48: PA4656; DOI: 10.1183/13993003.congress-2016.PA4656
Donato Lacedonia
1Medical and Surgical Sciences, University of Foggia, Foggia, Italy
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Grazia Pia Palladino
1Medical and Surgical Sciences, University of Foggia, Foggia, Italy
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Maria Pia Foschino Barbaro
1Medical and Surgical Sciences, University of Foggia, Foggia, Italy
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Cristina Colanardi
1Medical and Surgical Sciences, University of Foggia, Foggia, Italy
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Piera Soccio
1Medical and Surgical Sciences, University of Foggia, Foggia, Italy
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Incoronata Caccavo
1Medical and Surgical Sciences, University of Foggia, Foggia, Italy
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Giovanna E. Carpagnano
1Medical and Surgical Sciences, University of Foggia, Foggia, Italy
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Abstract

Introduction: The new phenotype of ACOS is described clinically as persistent airflow limitation combined with features of both asthma and COPD. miR-145 and miR-338 are involved in respectively in inflammation and tissue remodeling and their role was investigated in COPD and Asthma. The aim of this study was to evaluate the sputum level of miR-145 and miR-338 in ACOS patients compared with Asthma and COPD.

Methods: Sputum samples were obtained from 31 BPCO (yrs 70±8; FEV1% 59±23.4), 10 Asthma patients (yrs 52±14.3; FEV1% 93±11.8) and 6 ACOS (yrs 62±9.4; FEV1%74±10.3) selected according to Spanish guidelines. The RNA was isolated from supernatant of sputum with mirVana™ miRNA Isolation Kit (Ambion, Austin, TX) according to the manufacturer's protocol, the cDNA was processed using 7300 RealTime PCR. The data were than analyzed by ΔCt methods.

Results: Expression of miR-338 was lower in asthma group compared with COPD (-3.02±1.9 vs 0.22±2.4, p<0.01) while ACOS group shows an intermediate expression among two groups. miR-145 was positive expressed in all groups even if any changes were found among none of them. As expected, the percentage of neutrophils were higher in COPD (81±22.5) while higher level of eosinophils was found in ACOS group (21±34.4). miR-338 expression was positive correlated with neutrophils percentage (r=0.34, p=0.04).

Conclusion: This study provides the evidence that there is a different miRNA expression profile in sputum of COPD, ACOS and Asthma subjects.

  • COPD - mechanism
  • Asthma - mechanism
  • Molecular pathology
  • Copyright ©the authors 2016
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Sputum levels of miR-145 and miR-338 in COPD, asthma and ACOS subjects
Donato Lacedonia, Grazia Pia Palladino, Maria Pia Foschino Barbaro, Cristina Colanardi, Piera Soccio, Incoronata Caccavo, Giovanna E. Carpagnano
European Respiratory Journal Sep 2016, 48 (suppl 60) PA4656; DOI: 10.1183/13993003.congress-2016.PA4656

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Sputum levels of miR-145 and miR-338 in COPD, asthma and ACOS subjects
Donato Lacedonia, Grazia Pia Palladino, Maria Pia Foschino Barbaro, Cristina Colanardi, Piera Soccio, Incoronata Caccavo, Giovanna E. Carpagnano
European Respiratory Journal Sep 2016, 48 (suppl 60) PA4656; DOI: 10.1183/13993003.congress-2016.PA4656
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