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Comparison of genotype MTBDRplus assay for rapid detection of rifampicin and isoniazid resistance with conventional drug susceptibility tests in a tertiary care hospital in India

Madhav K. Kale, Parag Khatavkar, Pravin Dumne, Meenakshi Satpute, Nilima Telang
European Respiratory Journal 2016 48: PA2777; DOI: 10.1183/13993003.congress-2016.PA2777
Madhav K. Kale
1Chest, K.E.M. Hospital, Pune, MaharashtraIndia
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Parag Khatavkar
1Chest, K.E.M. Hospital, Pune, MaharashtraIndia
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Pravin Dumne
1Chest, K.E.M. Hospital, Pune, MaharashtraIndia
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Meenakshi Satpute
2Microbiology, K.E.M. Hopsital, Pune, MaharashtraIndia
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Nilima Telang
2Microbiology, K.E.M. Hopsital, Pune, MaharashtraIndia
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Abstract

INTRODUCTION: Emergence of drug resistant tuberculosis (TB) has necessitated the development of molecular based rapid diagnostic tests. WHO has approved Genotype MTBDRplus assay for detection of multidrug resistant (MDR) TB.

AIMS: To compare the performance of Genotype MTBDRplus assay with conventional drug susceptibility tests.

METHODS: Total 200 specimens (respiratory [sputum, bronchial lavage], lymph nodes, pleural fluid) were analysed on smear for AFB by ZN stain and Genotype MTBDRplus assay which was done as per manufacturer's instructions. Mycobacterium tuberculosis (MTB) cultures were done by radiometric method. Susceptibility tests were done on L.J. medium.

RESULTS:

1. Overall concordance of Rifampicin (RMP) and isoniazid (INH) results between Genotype MTBDRplus assay and conventional susceptibility results was 95.45% and 93.18% respectively.

2. Genotype MTBDRplus assay positivity in AFB smear negative and smear positive but MTB culture positive respiratory samples was 82.15% and 100% respectively.

MTB culture (Total 88)
Genotype MTBDRplus assayResistantSusceptibleSensitivity%Specificity%Positive predictive value%Negative predictive value%
RMP
Resistant210187.5098.4395.4595.45
Susceptible0363
INH
Resistant230595.8392.1882.1498.33
susceptible0159

3. Comparison of Genotype MTBDRplus assay with MTB cultures for RMP and INH resistance

CONCLUSIONS:

1. Genotype MTBDRplus assay is sensitive and specific for rapid diagnosis MDR TB .

2. The sensitivity is 100% in AFB smear positive respiratory samples.

3. Even in AFB smear negative pulmonary TB patients, assay is sensitive for diagnosis of MDR TB.

  • Tuberculosis - diagnosis
  • Tuberculosis - management
  • Copyright ©the authors 2016
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Comparison of genotype MTBDRplus assay for rapid detection of rifampicin and isoniazid resistance with conventional drug susceptibility tests in a tertiary care hospital in India
Madhav K. Kale, Parag Khatavkar, Pravin Dumne, Meenakshi Satpute, Nilima Telang
European Respiratory Journal Sep 2016, 48 (suppl 60) PA2777; DOI: 10.1183/13993003.congress-2016.PA2777

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Comparison of genotype MTBDRplus assay for rapid detection of rifampicin and isoniazid resistance with conventional drug susceptibility tests in a tertiary care hospital in India
Madhav K. Kale, Parag Khatavkar, Pravin Dumne, Meenakshi Satpute, Nilima Telang
European Respiratory Journal Sep 2016, 48 (suppl 60) PA2777; DOI: 10.1183/13993003.congress-2016.PA2777
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