Features of different M.tuberculosis strains that were isolated from MDR-TB patients in Kharkiv, Ukraine

Abstract

Multidrug-resistant tuberculosis (MDR TB) has found in 22 % new cases of tuberculosis (TB) and in 56% of retreated cases in Ukraine (WHO, 2015).

The aim: of the study was to estimate resistant of different M.tuberculosis (MBT) strains of MDR-TB patients.

Materials and methods: In 2014-2015, the 93 cases of MDR TB had studied in Kharkiv, Ukraine.

MBT identification and drug susceptibility testing (DST) had performed as recommended by WHO. VNTR genotyping was done by using sets of primers for amplification of five exact tandem repeats ETR loci (A, B, C, D, E).

Results and discussion: It was determined the variability of each locus by PCR from all 93 strains. By the VNTR-genotyping, the most common genotype was Beijing (69%). It was also demonstrated genotype LAM (13%), Africanum (3%) and Harlem (2%). 12 strains had not identified because of unique VNTR profiles.

Among the 93 patients with MDR-TB in 52 (56%) cases was found tuberculosis with enhanced resistance (XDR-TB).

There were no significant statistical differences in resistance frequency to the first-line anti-TB drugs among MBT strains (p>0.05). In 26 cases MBT from Beijing clade were resistant to all first-line anti-TB drugs.

In 69% and 60% cases respectively were found the resistance to Kanamycin (Km) and Ofloxacin (Ofx). The frequency of resistance to Km, Para-aminosalicilic acid (PAS) and Ethionamid (Et) was revealed significantly higher for LAM in compare to Beijing (p<0.05).

Conclusions: It is dificulte situation with MDR TB in Kharkiv region, Ukraine. Dominant strain of MDR TB is Bejing - 69% of cases. The resistance to the second-line anti-TB drugs revealed significantly higher for LAM strain in compare to Beijing strain.