The associations between a smoking and IL-10 gene polymorphism in patients with MDRTB

Abstract

Background and objective: Determine the associations between a smoking and IL-10 gene polymorphism in patients with MDRTB in lungs on the background of the respective IL-10 production of blood.

Methods: The study included 170 Caucasian people with infiltrative pulmonary TB permanently residing in Ukraine including 60 patients with MDRTB and smoking(1^stgroup),36-without MDRTB and smoking(2^ndgroup),14-with MDRTB and non-smoking(3^rdgroup )30-without MDRTB and non-smoking(4^thgroup)and 30 healthy donors (5^thgroup).Studied promoter region G1082A of IL-10 gene(rs1800896).Serum levels of IL-10 were evaluated by ELISA.

Results: In the 1^stgroup the levels of IL-10 were(31.11±1.16)pg/L,2^nd-(35.39±1.01)pg/L,3^rd-(38.27±0.88)pg/L,4^th-(42.39±0.92)and5^th-(50.25±1.26)pg/L(p<0.05). Homozygote GG genotype predominated in 5^thgroup was 56.67±9.05%(N=17) compared to patients with TB: 1^st group-3.33±2.32%(N=2), 2^nd-2.78±2.74%(N=1), 3^rd-7.14±7.14%(N=1) and 4^th-26.67±8.07%(N=8).Heterozygous GA genotype was observed in95.00±2.81%(N=57)in1^st,25.00±7.22%(N=9) in 2^nd,4.29±9.71%(N=2) in the 3^rd,6.67±4.55%(N=2) in 4^thand 23.33±7.72%(N=7) in 5^thgroups.Homozygous AA genotype was 1.67±1.65%(N=1) in the 1^stgroup,in 2^nd-72.22±7.47%(N=26), 3^rd-78.57±11.38%(N=11),4^th-66.67±8.61%(N=20)and5^th-20.00±7.30%(N=6)(p<0.05).

Conclusion: Smoking contributes to significant activation polymorphism G1082A gene IL-10 heterozygous GA genotype that may lead to changes in the immune system making susceptible to MDRTB.Compared to healthy controls patients with TB had significantly decreases levels of serum IL-10.This coincided with greater frequency of heterozygous GA and homozygous AA genotype polymorphism G1082A of the IL-10 gene.