Abstract
Background: ELISA assessment of cytokines (CK) plasma concentrations, particularly interleukins (IL) IL-6, IL-10, IL-18 was performed. The level of nitrogen monoxide (NO) defined the standard method by means Griess reagent.
Material and methods: The study included 63 patients with multidrug-resistant TB (MDR-TB).
Results: Assessment of IL-6 plasma concentration in pulmonary MDR-TB vs. sensible TB patients revealed a significant 1.7 folds increase (p<0.01), and, respectively, a significant 1.2 folds decrease in the level of IL-10 and IL-18 (p<0.001). Pearson correlation analysis between pro- and anti-inflammatory CK showed that in patients with MDR-TB there is a weak, negative correlation between the levels of IL-6 and IL-10 between IL-18 and IL-10 (r=-0.22, p<0,001, and r=-0.16, p<0.001, respectively). Investigation of NO contents showed, significant decline level of metabolites on 46 % in comparison to the norm (p<0,001), whith indicates the need for correction of the deficit arginine hydrochloride substrate, as is necessary for sufficient NO synthesis at prevalent tuberculous inflammation.
Conclusions: Comprehensive assessment level of pro- and anti-inflammatory CK level in MDR-TB patients showed a moderate endogenous intoxication, break down of the cellular component of the immune reactivity due to the formation conditions for the development of MTB resistance, because of cytotoxic hypoxia increase and activation of «systemic inflammatory response» syndrome. Analysis of plasma concentration of IL-6, IL-10 and IL-18 in patients with MDR-TB proved, that their level depends on the type of the resistance of MTB.
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