Assessment of anti-tuberculosis activity of extracts of cinnamomum verum and solanun surattense along with isoniazid

Abstract

Introduction: Tuberculosis(TB) remains a leading cause of mortality worldwide.Drug resistant TB have increased need for developing novel antituberculosis agents,using medicinal plants.

Aims and objectives: Study assessed antituberculosis and combined activity with Isoniazide(INH) of extracts of Cinnamomum verum & Solanum surattense against Mycobacterium tuberculosis H37Rv strain.

Methods: Aqueous & methanolic extracts was prepared by soxhet extraction and their phytochemical screening was done. Median Cytotoxicity (CC50) was estimated by MTT assay using cell line. Antituberculosis activity of extracts was tested by agar dilution & Microscopic Observation of Drug Susceptibility Assay (MODS).50% inhibition (IC50) was calculated by Resazurine Microtitre Assay (REMA).The combined effect of the extracts with INH was evaluated by checkerboard titrations.

Results: Phytochemical analysis showed flavanoids, tannins,steroids & essential-oils in both extracts. Minimum Inhibitory Concentration(MIC) of aqueous & methanolic extracts of both plants showed 10 mcg/ml by agar dilution method.Acquous (CAS) & methanolic(CMS) extracts of Cinnamomum showed MIC value of 0.5 & 2 mcg/ ml respectively, while aqueous (KAS) & methanolic (KMS) extracts of Solanum showed MIC value of 2 & 1 mcg/ ml resp. by MODS. IC 50 of CAS & CMS was 7.961 & 4.016 mcg/ ml respectively while IC50 of KAS & KMS was 11.27 & 8.5 mcg/ ml resp. by REMA. Antagonistic activity was observed in both plant extracts with INH.

Conclusion: Study demonstrated antituberculosis activity of plants,though antagonistic activity with INH.Active components in extracts need to be evaluated & standard testing protocol to be developed.