Abstract
Apelin is a novel endogenous peptide with inotropic and vasodilatory properties that is the ligand for the APJ receptor. The aim of this study was to investigate the relation of genetic variants on apelin with COPD in Turkish population. The present observational case–control study consisted of 341 Turkish subjects (224 COPD patients and 117 healthy controls), aged 40-80 years. Real-time polymerase chain reaction (RT-PCR) was used to analyze the 2 SNPs in COPD patients and healthy controls. Allele and genotype frequencies between patients and control subjects were compared using the chi-square (χ2) test.
| APLN1 rs3115758 Genotype | Control n (%) | COPD n (%) | P value |
| GG | 116(99.1) | 211(94.1) | *0,0353 |
| GT | 1(0.9) | 1(0.44) | |
| TT | 0(0) | 12(5.46) | |
| ALLELE | |||
| G | 233(99.58) | 425(94.42) | *0.0008 |
| T | 1(0.42) | 23(5.58) | |
| APLN1 rs3115759 Genotype | |||
| GG | 109(99.09) | 211(94.19) | *0.0419 |
| GA | 1(0.91) | 1(0.45) | |
| AA | 0(0) | 12(5.36) | |
| ALLELE | |||
| G | 423(94.42) | 219(99.55) | *0.0012 |
| A | 25(5.58) | 1(4.45) |
Genotypes and allele frequencies for APLN1 rs3115758 and APLN1 rs3115759 in COPD patients and controls
TT and AA risk genotypes of rs3115758 ve rs3115759 variants were significantly found to be related with risk of COPD with same power. The heterozygote and homozygote mutants genotypes of two variants (GT and TT and GA and AA respectively) were significantly found to be higher in patients group with the same rate. The homozygous TT genotype for rs3115758 increased the COPD risk with an OR 0,59 independent from other COPD risk factors.
This is the first study on Turkish population in literature which investigates the relationship between COPD and the variations of apelin gene. In this study, the variants on apelin gene were found to be related with COPD in Turkish population.
- Copyright ©the authors 2016
