Abstract
Aerobic training (AT) increases the expression of IL-10 that reduces airway inflammation; however, the origin of this anti-inflammatory cytokine is poorly known.
Aim: To investigate the expression of IL-10 by lung tissues and skeletal muscle in an asthma model.
Methods: 64 Balb/c mice were divided in groups: Control (CT), AT, Sensitized (OVA) or OVA+AT. Sensitized groups received inhalations (OVA 1-3%;3xwk;30min./session). AT was performed in a treadmill by 15 or 30 days (5xwk;60min./session;50% maximal exercise capacity). Outcomes: IgE and IgG1, bronchoalveolar cell counting as well IL-4, IL-12, TIMP-1, MMP-9, OPN, MCP-1 and anti-inflammatory cytokines (IL-10)(lung tissues). The expression of IL-10 was also quantified in the skeletal muscle (quadriceps).
Results: OVA groups had an increase in the IgE and IgG1, inflammatory cells and expression of all inflammatory cytokines compared with CT and AT groups (p<0.05). The OVA+AT groups had a reduction in the cell migration, IL-4, TIMP-1, MMP-9, OPN, MCP-1, and an increase of IL-10 expressed only by peribronchial cells (p<0.05). Curiously, AT only reduced the IgE, IgG1 and MCP-1 in OVA+AT group after 30 days (p<0.05). The expression of IL-10 by the quadriceps was not changed in either groups.
Conclusion: Aerobic conditioning increases the expression of IL-10 by peribronchial monocytes but not by peripheral muscles.
- Copyright ©ERS 2015
