Skip to main content

Main menu

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • Open access
    • COVID-19 submission information
    • Peer reviewer login
  • Alerts
  • Podcasts
  • Subscriptions
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

User menu

  • Log in
  • Subscribe
  • Contact Us
  • My Cart

Search

  • Advanced search
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

Login

European Respiratory Society

Advanced Search

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • Open access
    • COVID-19 submission information
    • Peer reviewer login
  • Alerts
  • Podcasts
  • Subscriptions

Enhanced neutrophil extracellular trap (NET) formation in sputum of stable COPD patients

Frauke Pedersen, Sebastian Marwitz, Torsten Goldmann, Anne Kirsten, Helgo Magnussen, Klaus F. Rabe, Mohib Uddin, Henrik Watz
European Respiratory Journal 2015 46: PA379; DOI: 10.1183/13993003.congress-2015.PA379
Frauke Pedersen
1Respiratory Medicine, LungClinic Grosshansdorf, Grosshansdorf, Germany
4Pulmonary Research Institute, LungClinic Grosshansdorf, Grosshansdorf, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sebastian Marwitz
2Clinical and Experimental Pathology, Research Center, Borstel, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Torsten Goldmann
2Clinical and Experimental Pathology, Research Center, Borstel, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Anne Kirsten
4Pulmonary Research Institute, LungClinic Grosshansdorf, Grosshansdorf, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Helgo Magnussen
4Pulmonary Research Institute, LungClinic Grosshansdorf, Grosshansdorf, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Klaus F. Rabe
1Respiratory Medicine, LungClinic Grosshansdorf, Grosshansdorf, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mohib Uddin
3Translational Science, Astra Zeneca, Mölndal, Sweden
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Henrik Watz
4Pulmonary Research Institute, LungClinic Grosshansdorf, Grosshansdorf, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
Loading

Abstract

COPD is characterized by neutrophilic airway inflammation. Neutrophil extracellular trap formation (NETosis), which consists of extracellular neutrophilic DNA, histones and granule enzymes, such as neutrophil elastase may regulate innate immune responses and potentially contribute to neutrophil-associated diseases. The presence of NETosis in sputum of stable COPD patients remains unknown. The aim of this study was to determine the level of NET formation in induced sputum from patients with COPD and to examine its relationship with sputum neutrophilia and airway obstruction. We evaluated induced sputum samples of 23 COPD patients (mean age, 67.0 ± 8.1 years; mean FEV1, 57.8 ± 16.6% pred.) and 10 healthy controls (mean age 55.0 ± 16.6 years) in whom airway obstruction were assessed using lung function testing. Sputum cells were harvested and stained with anti-histone H1, anti-neutrophil-elastase and DAPI. Concentration of extracellular DNA in sputum supernatants was quantified using a NanoDrop 2000 spectrophotometer. NETosis was markedly upregulated in COPD sputum compared with healthy controls as evaluated by immunofluorescence microscopy. NETosis was associated with significantly higher concentration of extracelluar DNA in sputum supernatant (mean 656.0 ± 894.6 ng/µL in COPD versus 300.2 ± 216.0 ng/µl in controls, p< 0.0001). Extracellular DNA correlated with absolute neutrophil numbers in sputum (r= 0.698; p< 0.0001) and airway obstruction (r=0.512; p=0.001). These results indicate that NETosis is markedly increased in the airways of stable COPD patients, suggesting that failure of timely clearance of NETosis may contribute to the unresolving neutrophilic airway inflammation in COPD.

  • Cell biology
  • COPD - mechanism
  • Inflammation
  • Copyright ©ERS 2015
Previous
Back to top
Vol 46 Issue suppl 59 Table of Contents
  • Table of Contents
  • Index by author
Email

Thank you for your interest in spreading the word on European Respiratory Society .

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Enhanced neutrophil extracellular trap (NET) formation in sputum of stable COPD patients
(Your Name) has sent you a message from European Respiratory Society
(Your Name) thought you would like to see the European Respiratory Society web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Enhanced neutrophil extracellular trap (NET) formation in sputum of stable COPD patients
Frauke Pedersen, Sebastian Marwitz, Torsten Goldmann, Anne Kirsten, Helgo Magnussen, Klaus F. Rabe, Mohib Uddin, Henrik Watz
European Respiratory Journal Sep 2015, 46 (suppl 59) PA379; DOI: 10.1183/13993003.congress-2015.PA379

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Enhanced neutrophil extracellular trap (NET) formation in sputum of stable COPD patients
Frauke Pedersen, Sebastian Marwitz, Torsten Goldmann, Anne Kirsten, Helgo Magnussen, Klaus F. Rabe, Mohib Uddin, Henrik Watz
European Respiratory Journal Sep 2015, 46 (suppl 59) PA379; DOI: 10.1183/13993003.congress-2015.PA379
del.icio.us logo Digg logo Reddit logo Technorati logo Twitter logo CiteULike logo Connotea logo Facebook logo Google logo Mendeley logo

Jump To

  • Article
  • Info & Metrics
  • Tweet Widget
  • Facebook Like
  • Google Plus One

More in this TOC Section

  • DP2/CRTh2 is expressed by ASM cells in asthma and its inhibition suppresses ASM migration
  • Micro-RNA-125a/b target A20 and MAVS to promote inflammatory and impair antiviral responses in chronic obstructive pulmonary disease
  • Steroid resistance in COPD is associated with impaired molecular chaperone HSP90 expression by pro-inflammatory lymphocytes
Show more 3.2 Airway Cell Biology and Immunopathology

Related Articles

Navigate

  • Home
  • Current issue
  • Archive

About the ERJ

  • Journal information
  • Editorial board
  • Reviewers
  • Press
  • Permissions and reprints
  • Advertising

The European Respiratory Society

  • Society home
  • myERS
  • Privacy policy
  • Accessibility

ERS publications

  • European Respiratory Journal
  • ERJ Open Research
  • European Respiratory Review
  • Breathe
  • ERS books online
  • ERS Bookshop

Help

  • Feedback

For authors

  • Instructions for authors
  • Publication ethics and malpractice
  • Submit a manuscript

For readers

  • Alerts
  • Subjects
  • Podcasts
  • RSS

Subscriptions

  • Accessing the ERS publications

Contact us

European Respiratory Society
442 Glossop Road
Sheffield S10 2PX
United Kingdom
Tel: +44 114 2672860
Email: journals@ersnet.org

ISSN

Print ISSN:  0903-1936
Online ISSN: 1399-3003

Copyright © 2023 by the European Respiratory Society