Abstract
Introduction: Pulmonary sarcoidosis is an inflammatory granulomatous disease with the deregulated expression profile of intracellular miRNAs. There has been however the limited information on exosomal miRNAs in sarcoidosis and no data on either bronchoalveolar (BAL) miRNAs or their possible changes during the disease progression exist.
Aim: To assess exosomal expressions of 27 BAL and 32 serum miRNAs in our sarcoid patients with different disease stages. Method: Multiplex real-time PCR with TaqMan assays was used to assess the relative expressions of the selected miRNAs in serum obtained from 13 sarcoid patients with Lofgren syndrome (LS) and from 13 with advanced CXR (chest X-ray) stage ≥3 and in BAL fluid from 23 CXR stage I (11 LS and 12 without LS) and 12 CXR stage ≥3. The internal controls among exosomal miRNAs were selected by NormFinder and GeNorm algorithms.
Results: Of individual miRNAs, serum miR-425 and BAL miR-17 had the lowest expression variability. Global geometric means showed the best stability in serum and BAL fluid. The elevated serum expression of miR-340 (p<0.01) and decreased serum miR-212 and miR-21 were associated with sarcoidosis progression (p<0.02 and p<0.03) . None of BAL miRNAs was deregulated in our sarcoid patients (p>0.05). Conclusion: We showed three exosomal miRNAs (miR-340, miR-212 and miR-21) to be deregulated in sarcoidosis course. Grant support:CZ.1.07/2.3.00/30.0004,LO1304,IGA UP: LF_2015_020.
- Copyright ©ERS 2015