Abstract
Introduction: Nasal discharge is very common and has significant impact on quality of life. Several therapies are available, but none has the aim to increase nasal mucociliary clearance by enhancing ciliary activity.
Aim: We aimed to study the influence of a new compound for nasal use, Ciliactive®, on ciliary activity in vitro.
Methods: Human nasal epithelial cells expressing motile cilia were obtained from surgery specimens and cultured as a monolayer. We studied the influence of Ciliactive® (C), compared to saline (NaCl 0,9%, S). The compounds were diluted in cell culture medium and ciliary beat frequency (CBF) was followed during exposure (0, 1, 5, 24, 48 hours). CBF was measured with a high speed camera, mounted on an inverted microscope: each result was the mean of 5 measurements. All results were expressed as % of the starting value. C and S were used in concentrations of 50, 75 and 100 %, diluted in cell culture medium. Conditions were compared using paired t-tests.
Results: Samples from 8 persons were included. C significantly increased CBF in all concentrations, compared to the paired controls with S (p<0.005 for 5h, p<0.002 for 24h, p<0.03 for 48h). C increased CBF with 15, 24 and 15% after 24h, compared to S for a concentration of 50%, 75% and 100 % respectively.
Conclusion: Ciliactive® is a new compound, that enhances ciliary activity in vitro. It might be useful to treat nasal mucociliary clearance disorders.
- Copyright ©ERS 2015
