Skip to main content

Main menu

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • Open access
    • COVID-19 submission information
    • Peer reviewer login
  • Alerts
  • Podcasts
  • Subscriptions
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

User menu

  • Log in
  • Subscribe
  • Contact Us
  • My Cart

Search

  • Advanced search
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

Login

European Respiratory Society

Advanced Search

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • Open access
    • COVID-19 submission information
    • Peer reviewer login
  • Alerts
  • Podcasts
  • Subscriptions

Placenta-derived conditioned medium with anti-tumor properties on human NSCLC

Dagmar S. Lang, Sebastian Marwitz, Tobias Zeiser, Sophie Seehase, Iris Watermann, Ekkehard Vollmer, Martin Reck, Peter Zabel, Torsten Goldmann
European Respiratory Journal 2015 46: OA4983; DOI: 10.1183/13993003.congress-2015.OA4983
Dagmar S. Lang
1Clinical and Experimental Pathology, Research Center Borstel, Borstel, Germany
5Airway Research Center North (ARCN), Member of the German Center for Lung Research (DZL), Borstel, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sebastian Marwitz
1Clinical and Experimental Pathology, Research Center Borstel, Borstel, Germany
5Airway Research Center North (ARCN), Member of the German Center for Lung Research (DZL), Borstel, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tobias Zeiser
2Gynecology and Obstetrics, Paracelsus Clinic, Henstedt-Ulzburg, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sophie Seehase
1Clinical and Experimental Pathology, Research Center Borstel, Borstel, Germany
5Airway Research Center North (ARCN), Member of the German Center for Lung Research (DZL), Borstel, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Iris Watermann
3Oncology, LungenClinic Grosshansdorf, Grosshansdorf, Germany
5Airway Research Center North (ARCN), Member of the German Center for Lung Research (DZL), Borstel, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ekkehard Vollmer
1Clinical and Experimental Pathology, Research Center Borstel, Borstel, Germany
5Airway Research Center North (ARCN), Member of the German Center for Lung Research (DZL), Borstel, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Martin Reck
3Oncology, LungenClinic Grosshansdorf, Grosshansdorf, Germany
5Airway Research Center North (ARCN), Member of the German Center for Lung Research (DZL), Borstel, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Peter Zabel
4Medicine, Research Center Borstel, Borstel, Germany
5Airway Research Center North (ARCN), Member of the German Center for Lung Research (DZL), Borstel, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Torsten Goldmann
1Clinical and Experimental Pathology, Research Center Borstel, Borstel, Germany
5Airway Research Center North (ARCN), Member of the German Center for Lung Research (DZL), Borstel, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
Loading

Abstract

The decidua of human term placenta contains a cell population with highly immunomodulatory properties regulating viability, growth and invasion important for the fetal development, remarkably resembling the tumor-microenvironment. Prompted by previously published data from our group demonstrating the pro-apoptotic potential of placenta-derived supernatant on human lung cancer in vitro, the anti-tumor activity of placenta-conditioned medium was studied in more detail. For this purpose, human NSCLC cell lines A549 and H838 were challenged for 17h with placenta-derived conditioned medium, obtained from human fresh term placenta incubated overnight in DMEM with 2%FCS.

Viability was analysed either by MTT assay or FACS using 7-AAD. For tumor growth, expression of proliferation marker Ki-67 was analysed, induction of apoptosis was determined by expression of annexin V and/or cleaved caspase 3 using immunofluorescence or FACS analysis. Overall, both suppression of Ki-67 expression and increase of apoptotic cells were induced by all placentas (n=6), although to strikingly varying extent. Based on these preliminary but promising results demonstrating consistent inhibitory effects on lung tumor cells, placenta-derived anti-tumor activity will be further studied including human NSCLC specimens and by using fractionated placenta-derived supernatants that will be also analysed by mass spectrometry to identify potential immunosuppressive mediators. Thus, these studies could result in the development of new therapeutic agents for a more effective treatment of NSCLC.

  • Lung cancer / Oncology
  • Immunosuppression
  • Cell biology
  • Copyright ©ERS 2015
Previous
Back to top
Vol 46 Issue suppl 59 Table of Contents
  • Table of Contents
  • Index by author
Email

Thank you for your interest in spreading the word on European Respiratory Society .

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Placenta-derived conditioned medium with anti-tumor properties on human NSCLC
(Your Name) has sent you a message from European Respiratory Society
(Your Name) thought you would like to see the European Respiratory Society web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Placenta-derived conditioned medium with anti-tumor properties on human NSCLC
Dagmar S. Lang, Sebastian Marwitz, Tobias Zeiser, Sophie Seehase, Iris Watermann, Ekkehard Vollmer, Martin Reck, Peter Zabel, Torsten Goldmann
European Respiratory Journal Sep 2015, 46 (suppl 59) OA4983; DOI: 10.1183/13993003.congress-2015.OA4983

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Placenta-derived conditioned medium with anti-tumor properties on human NSCLC
Dagmar S. Lang, Sebastian Marwitz, Tobias Zeiser, Sophie Seehase, Iris Watermann, Ekkehard Vollmer, Martin Reck, Peter Zabel, Torsten Goldmann
European Respiratory Journal Sep 2015, 46 (suppl 59) OA4983; DOI: 10.1183/13993003.congress-2015.OA4983
del.icio.us logo Digg logo Reddit logo Technorati logo Twitter logo CiteULike logo Connotea logo Facebook logo Google logo Mendeley logo

Jump To

  • Article
  • Info & Metrics
  • Tweet Widget
  • Facebook Like
  • Google Plus One

More in this TOC Section

  • Early surgical resection for stage I high-grade neuroendocrine caricinoma of lung
  • Checkpointinhibition in lung cancer – How to distinguish pseudoprogression from progressive disease
  • LATE-BREAKING ABSTRACT: Early relapse of non-small cell lung cancer (NSCLC) found after CNS-symptoms
Show more 11.1 Lung Cancer

Related Articles

Navigate

  • Home
  • Current issue
  • Archive

About the ERJ

  • Journal information
  • Editorial board
  • Reviewers
  • Press
  • Permissions and reprints
  • Advertising

The European Respiratory Society

  • Society home
  • myERS
  • Privacy policy
  • Accessibility

ERS publications

  • European Respiratory Journal
  • ERJ Open Research
  • European Respiratory Review
  • Breathe
  • ERS books online
  • ERS Bookshop

Help

  • Feedback

For authors

  • Instructions for authors
  • Publication ethics and malpractice
  • Submit a manuscript

For readers

  • Alerts
  • Subjects
  • Podcasts
  • RSS

Subscriptions

  • Accessing the ERS publications

Contact us

European Respiratory Society
442 Glossop Road
Sheffield S10 2PX
United Kingdom
Tel: +44 114 2672860
Email: journals@ersnet.org

ISSN

Print ISSN:  0903-1936
Online ISSN: 1399-3003

Copyright © 2023 by the European Respiratory Society