Abstract
Telomere biology has been extensively studied in lung adenocarcinomas, but the results so far are controversial and scarce. We aimed at inferring telomere length associated gene regulatory network in lung adenocarcinoma, based on next generation whole genome (WGS) and transcriptome (RNAseq) sequencing data.
Paired WGS and RNAseq data for 26 lung adenocarcinoma cell lines were obtained from (Suzuki, A, Nucleic Acids Res 2014; 42(22):13557-72). Telomere length was calculated from WGS data using in-house software Computel. A partial correlation gene network was built with R package “GeneNet”, and filtered for edges with correlation values less than 0.1. The resulting network revealed partial correlations between telomere length and five genes (Figure 1).
From these genes, the PIR and GPR19 were previously implicated in lung cancer by helping to overcome the senescence barrier and acceleration of mesenchymal transition through cell-cycle. ASUN, FECH and NDUFA1 has not been widely investigated in lung cancers, however, they might have a role in control of cell cycle and mitochondrial processes.
In conclusion, we have identified genes, which either regulated or are regulated by telomere length dynamics in lung adenocarcinoma cell lines. Investigation of their role in the disease development may provide new insights into dynamics of lung cancer cells.
- Copyright ©ERS 2015
