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Early life gut microbial alterations in children diagnosed with asthma by three years of age

Leah Stiemsma, Marie-Claire Arrieta, Pedro Dimitriu, Lisa Thorson, Sophie Yurist-Doutsch, Boris Kuzeljevic, Diana Lefebvre, Padmaja Subbarao, Piush Mandhane, Allan Becker, Malcolm Sears, Kelly McNagny, Tobias Kollmann, The Child Study Investigators, William Mohn, Brett Finlay, Stuart Turvey
European Respiratory Journal 2015 46: OA1971; DOI: 10.1183/13993003.congress-2015.OA1971
Leah Stiemsma
1Microbiology & Immunology, University of British Columbia, Vancouver, BC Canada
2Pediatrics, Child & Family Research Institute, Vancouver, BC Canada
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Marie-Claire Arrieta
1Microbiology & Immunology, University of British Columbia, Vancouver, BC Canada
3Michael Smith Laboratories, University of British Columbia, Vancouver, BC Canada
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Pedro Dimitriu
1Microbiology & Immunology, University of British Columbia, Vancouver, BC Canada
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Lisa Thorson
3Michael Smith Laboratories, University of British Columbia, Vancouver, BC Canada
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Sophie Yurist-Doutsch
3Michael Smith Laboratories, University of British Columbia, Vancouver, BC Canada
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Boris Kuzeljevic
2Pediatrics, Child & Family Research Institute, Vancouver, BC Canada
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Diana Lefebvre
4Medicine, St. Joseph's Healthcare, Hamilton, ON Canada
5Medicine, McMaster University, Hamilton, BC Canada
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Padmaja Subbarao
6Pediatrics, University of Toronto, Toronto, ON Canada
7Respiratory Medicine, Hospital for Sick Children, Toronto, ON Canada
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Piush Mandhane
8Pediatrics, University of Alberta, Edmonton, AB Canada
9School of Public Health, University of Alberta, Edmonton, AB Canada
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Allan Becker
10Pediatrics and Child Health, University of Manitoba, Winnipeg, MB Canada
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Malcolm Sears
4Medicine, St. Joseph's Healthcare, Hamilton, ON Canada
5Medicine, McMaster University, Hamilton, BC Canada
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Kelly McNagny
11Biomedical Research Centre, University of British Columbia, Vancouver, BC Canada
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Tobias Kollmann
2Pediatrics, Child & Family Research Institute, Vancouver, BC Canada
12Pediatrics, University of British Columbia, Vancouver, BC Canada
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1Microbiology & Immunology, University of British Columbia, Vancouver, BC Canada
William Mohn
1Microbiology & Immunology, University of British Columbia, Vancouver, BC Canada
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Brett Finlay
1Microbiology & Immunology, University of British Columbia, Vancouver, BC Canada
3Michael Smith Laboratories, University of British Columbia, Vancouver, BC Canada
13Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC Canada
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Stuart Turvey
2Pediatrics, Child & Family Research Institute, Vancouver, BC Canada
12Pediatrics, University of British Columbia, Vancouver, BC Canada
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Abstract

Background: Previously our group associated early life gut microbial alterations in one-year-old children with risk of active asthma at school age. These early life alterations were characterized by significant reductions in four bacterial genera and a decreased production of microbial derived metabolites. We hypothesize that this early life gut microbial dysbiotic state is also associated with asthma diagnosed in children by three years of age and aim to identify additional bacterial populations that might be contributing to this asthmatic phenotype.

Methods: 286 children enrolled in the Canadian Healthy Infant Longitudinal Development (CHILD) study were classified according to the diagnosis of asthma by three years of age. Bacterial 16S rDNA from 3-month and 1-year stool samples from these children was extracted, amplified, and subjected to high throughput Illumina sequencing. Bacterial genera were quantified by quantitative PCR in 16S rDNA samples from all asthmatic children and a subset of controls. An exact logistic regression model was used to assess the effects of potentially confounding variables (i.e. antibiotic exposure, mode of birth).

Results: 16S sequence analysis of our sample cohort (286 subjects) identified differentially abundant bacterial populations between asthmatics and controls at 3-months of age. Additionally, qPCR identified significant shifts in the abundance of specific bacterial genera at 3-months and 1-year of age in the asthmatic group when compared to controls.

Conclusions: Shifts in the relative abundance of certain gut bacterial populations in early life are associated with asthma diagnosed in children by three years of age.

  • Asthma - mechanism
  • Bacteria
  • Biomarkers
  • Copyright ©ERS 2015
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Early life gut microbial alterations in children diagnosed with asthma by three years of age
Leah Stiemsma, Marie-Claire Arrieta, Pedro Dimitriu, Lisa Thorson, Sophie Yurist-Doutsch, Boris Kuzeljevic, Diana Lefebvre, Padmaja Subbarao, Piush Mandhane, Allan Becker, Malcolm Sears, Kelly McNagny, Tobias Kollmann, The Child Study Investigators, William Mohn, Brett Finlay, Stuart Turvey
European Respiratory Journal Sep 2015, 46 (suppl 59) OA1971; DOI: 10.1183/13993003.congress-2015.OA1971

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Early life gut microbial alterations in children diagnosed with asthma by three years of age
Leah Stiemsma, Marie-Claire Arrieta, Pedro Dimitriu, Lisa Thorson, Sophie Yurist-Doutsch, Boris Kuzeljevic, Diana Lefebvre, Padmaja Subbarao, Piush Mandhane, Allan Becker, Malcolm Sears, Kelly McNagny, Tobias Kollmann, The Child Study Investigators, William Mohn, Brett Finlay, Stuart Turvey
European Respiratory Journal Sep 2015, 46 (suppl 59) OA1971; DOI: 10.1183/13993003.congress-2015.OA1971
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More in this TOC Section

  • Asthma phenotypes in children: Allergic versus non allergic
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Show more 7.2 Paediatric Asthma and Allergy

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