Abstract
Introduction
The Ion Mobility Spectrometry (IMS) is a highly sensitive analytical method for detection of volatile organic compounds (VOCs) in air. Available studies were typically done with one device in mono-centric studies only. The aim of the study was the comparison of different devices.
Methods:
The headspaces of in vitro cultures of two strains of mycobacterium avium were measured with a GC-differential-ion-mobility-spectrometry (DMS-SIONEX) as well as with a GC-IMS Prototype (GC-IMS) 1 week after inoculation. Pure breeding grounds were measured as control. Both devices show spectra with comparable numbers of peaks. The spectra were analyzed by a statistical program based on cluster analysis.
Results
With both devices was possible a significant differentiation between control and the bacterial growth. Each cluster represents the same peaks, e.g. detected metabolite, in the different measurements. Fig. 1 shows clusters for differentiation between control (left) and strain 1 (middle) and strain 2 (right) for both devices.
Discussion
With both devices the differentiation between breeding ground and bacterial growth was possible with a sensitivity and specifity of 100%. The detected clusters should be verified between the devices. Differentiation between two devices will be helpful for evaluation of peaks. GC-IMS may be a more rapid tool for detection of bacterial growth in vitro than traditional methods.
- © 2014 ERS
