Abstract
Background: Lung transplantation has been the last therapeutic management for patients with end-stage lung disease. The organ donors are frequently excluded because of the underlying diseases such as liver cirrhosis, infectious diseases, chronic renal failure (CRF) etc. which probably related to the intolerance to ischemia/reperfusion (IR) injury. The degree of CRF injured lungs during IR is still unclear.
Aims: To determine the effect of I/R-induced acute lung injury in CRF rats.
Materials and Methods: Spraque-Dawley rats were fed with 0.75% adenine chow to trigger CRF. We applied in situ isolated lung model to evaluate ischemia–reperfusion -induced acute lung injury. Animals were divided into a control group, ischemia–reperfusion group, and renal failure with IR groups.
Results: Ischemia/reperfusion caused significant increases in alveolar lavage and perfusate tumor necrosis factor, inflammatory cell infiltration, lung tissue malondialdehyde, bronchoalveolar lavage fluid protein concentration and lactate dehydrogenase activity, lung weight gain, and infiltration coefficient. Adenine-induced renal failure significantly suppressed the inflammatory response and attenuated IR lung injury. Our results also revealed less severity of ischemia- reperfusion-induced phosphorylation and nuclear translocation of nuclear factor-κB in rats with that adenine-induced renal failure which was associated with reduced IκB kinase (IKK)-β phosphorylation, suggesting a suppression of IκB kinase and thus IκB-κ activation.
Conclusion: Our findings suggest that CRF rats might prevent the lungs from IR-induced lung injury probably related to the self-protection mechanism by chronic renal failure.
- © 2014 ERS
