Skip to main content

Main menu

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • ERS Guidelines
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • Open access
    • Peer reviewer login
  • Alerts
  • Subscriptions
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

User menu

  • Log in
  • Subscribe
  • Contact Us
  • My Cart

Search

  • Advanced search
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

Login

European Respiratory Society

Advanced Search

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • ERS Guidelines
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • Open access
    • Peer reviewer login
  • Alerts
  • Subscriptions

The effect of smoking on phosphoinositide 3-kinase (PI3K) and phosphatase and tensin homolog deleted from chromosome 10 (PTEN) mRNA expression in human airway epithelial cells

Kazuhiro Yamada, Kazuhisa Asai, Yuko Ohara, Yukari Sugiyama, Atsuko Shirai, Kanako Sato, Norio Yamamoto, Gakuya Tamagaki, Tetsuya Watanabe, Kazuhisa Konishi, Yoshihiro Tochino, Masato Uji, Hiroshi Kanazawa, Kazuto Hirata
European Respiratory Journal 2014 44: P3900; DOI:
Kazuhiro Yamada
1Respiratory Medicine, Osaka City University, Osaka, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kazuhisa Asai
1Respiratory Medicine, Osaka City University, Osaka, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yuko Ohara
1Respiratory Medicine, Osaka City University, Osaka, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yukari Sugiyama
1Respiratory Medicine, Osaka City University, Osaka, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Atsuko Shirai
1Respiratory Medicine, Osaka City University, Osaka, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kanako Sato
1Respiratory Medicine, Osaka City University, Osaka, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Norio Yamamoto
1Respiratory Medicine, Osaka City University, Osaka, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gakuya Tamagaki
1Respiratory Medicine, Osaka City University, Osaka, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tetsuya Watanabe
1Respiratory Medicine, Osaka City University, Osaka, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kazuhisa Konishi
1Respiratory Medicine, Osaka City University, Osaka, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yoshihiro Tochino
1Respiratory Medicine, Osaka City University, Osaka, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Masato Uji
1Respiratory Medicine, Osaka City University, Osaka, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hiroshi Kanazawa
1Respiratory Medicine, Osaka City University, Osaka, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kazuto Hirata
1Respiratory Medicine, Osaka City University, Osaka, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
Loading

Abstract

Introduction

Cigarette smoking, a major etiology of chronic obstructive pulmonary disease (COPD) , causes persistent airway inflammation.Phosphoinositide 3-kinases (PI3Ks) are family of enzyme involved in synthesis of phosphatidylinositol (3,4,5)-triphosphate (PtdIns(3,4,5)P3), which induce cellular functions such as cell growth, proliferation, differentiation and production of inflammatory mediator. In contrast, Phosphatase and Tensin Homolog Deleted from Chromosome 10 (PTEN) is known to be an endogenous inhibitor of PI3Ks. However, little is known regarding the effect of cigarette smoke on PI3Ks and PTEN expression.

Aim

The aim of this study was to clarify the effect of cigarette smoke on PI3Ks and PTEN expression in human airway epithelial cells.

Methods

Bronchoscopic brushing cells from twenty current smokers (15 COPD patients and 5 smoking non-COPD patients) and 7 healthy non-smoker were evaluated for PI3Kcatalytic delta polypeptide (PI3KCD) and PTEN mRNA expressions by RT-PCR.

Results

The ratio of PI3KCD to PTEN mRNA expression was significantly increased (p<0.05), while there were trends towards increased PI3KCD mRNA expression and decreased PTEN mRNA expression in smokers. Expression of PTEN mRNA in smokers was significantly correlated with Brinkman Index (r=-0.52, p<0.05).

Conclusion

Cigarette smoke altered PI3KCD/PTEN balance in human airway epithelial cells.

  • COPD - mechanism
  • Bronchoscopy
  • Epithelial cell
  • © 2014 ERS
Previous
Back to top
Vol 44 Issue Suppl 58 Table of Contents
  • Table of Contents
  • Index by author
Email

Thank you for your interest in spreading the word on European Respiratory Society .

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
The effect of smoking on phosphoinositide 3-kinase (PI3K) and phosphatase and tensin homolog deleted from chromosome 10 (PTEN) mRNA expression in human airway epithelial cells
(Your Name) has sent you a message from European Respiratory Society
(Your Name) thought you would like to see the European Respiratory Society web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
The effect of smoking on phosphoinositide 3-kinase (PI3K) and phosphatase and tensin homolog deleted from chromosome 10 (PTEN) mRNA expression in human airway epithelial cells
Kazuhiro Yamada, Kazuhisa Asai, Yuko Ohara, Yukari Sugiyama, Atsuko Shirai, Kanako Sato, Norio Yamamoto, Gakuya Tamagaki, Tetsuya Watanabe, Kazuhisa Konishi, Yoshihiro Tochino, Masato Uji, Hiroshi Kanazawa, Kazuto Hirata
European Respiratory Journal Sep 2014, 44 (Suppl 58) P3900;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
The effect of smoking on phosphoinositide 3-kinase (PI3K) and phosphatase and tensin homolog deleted from chromosome 10 (PTEN) mRNA expression in human airway epithelial cells
Kazuhiro Yamada, Kazuhisa Asai, Yuko Ohara, Yukari Sugiyama, Atsuko Shirai, Kanako Sato, Norio Yamamoto, Gakuya Tamagaki, Tetsuya Watanabe, Kazuhisa Konishi, Yoshihiro Tochino, Masato Uji, Hiroshi Kanazawa, Kazuto Hirata
European Respiratory Journal Sep 2014, 44 (Suppl 58) P3900;
del.icio.us logo Digg logo Reddit logo Technorati logo Twitter logo CiteULike logo Connotea logo Facebook logo Google logo Mendeley logo

Jump To

  • Article
  • Info & Metrics
  • Tweet Widget
  • Facebook Like
  • Google Plus One

More in this TOC Section

  • Micro-RNA-125a/b target A20 and MAVS to promote inflammatory and impair antiviral responses in chronic obstructive pulmonary disease
  • High-fat diet in mice leads to amplified ozone-induced airway hyperresponsiveness (AHR), mitochondrial dysfunction and insulin resistance
  • Inhibition of asthma-related immunological responses in mast cells and Th2 cell lines by cultured epithelial cell lines
Show more 3.2 Airway Cell Biology and Immunopathology

Related Articles

Navigate

  • Home
  • Current issue
  • Archive

About the ERJ

  • Journal information
  • Editorial board
  • Press
  • Permissions and reprints
  • Advertising

The European Respiratory Society

  • Society home
  • myERS
  • Privacy policy
  • Accessibility

ERS publications

  • European Respiratory Journal
  • ERJ Open Research
  • European Respiratory Review
  • Breathe
  • ERS books online
  • ERS Bookshop

Help

  • Feedback

For authors

  • Instructions for authors
  • Publication ethics and malpractice
  • Submit a manuscript

For readers

  • Alerts
  • Subjects
  • Podcasts
  • RSS

Subscriptions

  • Accessing the ERS publications

Contact us

European Respiratory Society
442 Glossop Road
Sheffield S10 2PX
United Kingdom
Tel: +44 114 2672860
Email: journals@ersnet.org

ISSN

Print ISSN:  0903-1936
Online ISSN: 1399-3003

Copyright © 2023 by the European Respiratory Society