Skip to main content

Main menu

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • ERS Guidelines
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • Open access
    • Peer reviewer login
  • Alerts
  • Subscriptions
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

User menu

  • Log in
  • Subscribe
  • Contact Us
  • My Cart

Search

  • Advanced search
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

Login

European Respiratory Society

Advanced Search

  • Home
  • Current issue
  • ERJ Early View
  • Past issues
  • ERS Guidelines
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • Open access
    • Peer reviewer login
  • Alerts
  • Subscriptions

Mass spectrometry methods for biomarker research for chronic lung allograft dysfunction (CLAD) in systems prediction of CLAD (SysCLAD)

Johanna Claustre, Sébastien Quétant, Candice Trocmé, Sandrine Bourgoin-Voillard, Hélène Flamant-Waret, Izabel Bérard, Bertrand Toussaint, Karine Botturi-Cavaillès, Antoine Magnan, Laurent Nicod, Christophe Pison, Michel Sève
European Respiratory Journal 2014 44: P2457; DOI:
Johanna Claustre
1Clinique universitaire de Pneumologie, CHU de Grenoble, Grenoble, France
2Plateforme de Protéomique Prométhée, INSERM U823 - IAB, Grenoble, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sébastien Quétant
1Clinique universitaire de Pneumologie, CHU de Grenoble, Grenoble, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Candice Trocmé
3Laboratoire de Biochimie des Enzymes et des Protéines, CHU de Grenoble, Grenoble, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sandrine Bourgoin-Voillard
2Plateforme de Protéomique Prométhée, INSERM U823 - IAB, Grenoble, France
4Université Joseph Fourier, UJF, Grenoble, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hélène Flamant-Waret
2Plateforme de Protéomique Prométhée, INSERM U823 - IAB, Grenoble, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Izabel Bérard
2Plateforme de Protéomique Prométhée, INSERM U823 - IAB, Grenoble, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bertrand Toussaint
3Laboratoire de Biochimie des Enzymes et des Protéines, CHU de Grenoble, Grenoble, France
4Université Joseph Fourier, UJF, Grenoble, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Karine Botturi-Cavaillès
5Service de Pneumologie, CHU de Nantes, Nantes, France
6Institut du Thorax, INSERM UMR1087/CNRS UMR6291, Nantes, France
7DHU 2020, Université de Nantes, Nantes, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Antoine Magnan
5Service de Pneumologie, CHU de Nantes, Nantes, France
6Institut du Thorax, INSERM UMR1087/CNRS UMR6291, Nantes, France
7DHU 2020, Université de Nantes, Nantes, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Laurent Nicod
8Service de Pneumologie, Centre Hospitalo universitaire Vaudois, Lausanne, Switzerland
9Université de Lausanne, UNIL, Lausanne, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Christophe Pison
1Clinique universitaire de Pneumologie, CHU de Grenoble, Grenoble, France
4Université Joseph Fourier, UJF, Grenoble, France
10Laboratoire de Bioloénergétique Fondamentale et Appliquée, INSERM 1055, Grenoble, France
11European Institute of Systems Biology and Medicine, EISBM, Lyon, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michel Sève
2Plateforme de Protéomique Prométhée, INSERM U823 - IAB, Grenoble, France
4Université Joseph Fourier, UJF, Grenoble, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
Loading

Abstract

Background: CLAD is an irreversible and heterogeneous condition, associating Bronchiolitis Obliterans Syndrome (BOS), and Restrictive Allograft Syndrome (RAS). RAS and early onset of BOS are correlated with a poor prognosis and a significant morbidity in lung transplant recipients. As a part of SysCLAD, a FP-7 funded project aimed to predict CLAD, this study was designed to find biomarkers of CLAD in lung transplant recipients from the French cohort of lung transplant COLT and the Swiss cohort STCS, by using 2 mass spectrometry (MS) techniques.

Methods: BALF and plasma in the 200 first patients of COLT cohort who reached 3 years post-transplantation or developed an early form of CLAD were selected for analysis from systematic visits at months 6 and 12. First, SELDI-TOF MS analysis, a high-throughput method, allowed obtaining individual proteomic profiles using 3 complementary arrays: CM10 (cation array), Q10 (anion array) and IMAC-Cu (copper array). Furthermore, after pooling samples according to patients' phenotype (BOS, RAS, stable), iTRAQ-MALDI-TOF MS and MS/MS analysis, a sensitive and quantitative method, allowed biomarker identification after peptide separation by off-gel fractionation and nano-reversed phase chromatography.

Results: In a pilot study, 45 proteins were differentially expressed in BALF with SELDI-TOF MS analysis. Identification and quantification of these potential biomarkers is currently performed with iTRAQ analysis.

Conclusion: Identification and validation of early biomarkers of CLAD with these two complementary methods may help to understand involved mechanisms and to earlier diagnose CLAD onset at a reversible stage.

  • Transplantation
  • Biomarkers
  • Proteomics
  • © 2014 ERS
Previous
Back to top
Vol 44 Issue Suppl 58 Table of Contents
  • Table of Contents
  • Index by author
Email

Thank you for your interest in spreading the word on European Respiratory Society .

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Mass spectrometry methods for biomarker research for chronic lung allograft dysfunction (CLAD) in systems prediction of CLAD (SysCLAD)
(Your Name) has sent you a message from European Respiratory Society
(Your Name) thought you would like to see the European Respiratory Society web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Mass spectrometry methods for biomarker research for chronic lung allograft dysfunction (CLAD) in systems prediction of CLAD (SysCLAD)
Johanna Claustre, Sébastien Quétant, Candice Trocmé, Sandrine Bourgoin-Voillard, Hélène Flamant-Waret, Izabel Bérard, Bertrand Toussaint, Karine Botturi-Cavaillès, Antoine Magnan, Laurent Nicod, Christophe Pison, Michel Sève
European Respiratory Journal Sep 2014, 44 (Suppl 58) P2457;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Mass spectrometry methods for biomarker research for chronic lung allograft dysfunction (CLAD) in systems prediction of CLAD (SysCLAD)
Johanna Claustre, Sébastien Quétant, Candice Trocmé, Sandrine Bourgoin-Voillard, Hélène Flamant-Waret, Izabel Bérard, Bertrand Toussaint, Karine Botturi-Cavaillès, Antoine Magnan, Laurent Nicod, Christophe Pison, Michel Sève
European Respiratory Journal Sep 2014, 44 (Suppl 58) P2457;
Reddit logo Technorati logo Twitter logo Connotea logo Facebook logo Mendeley logo

Jump To

  • Article
  • Info & Metrics
  • Tweet Widget
  • Facebook Like
  • Google Plus One

More in this TOC Section

  • Risk of post-transplantation diabetes mellitus (PTDM) with different calcineurin inhibitors
  • Utility of C4d by immunohistochemistry for the diagnosis of antibody-mediated rejection in lung transplantation
  • Inflammation and primary graft dysfunction after lung transplantation: CT-PET findings
Show more 8.2 Transplantation

Related Articles

Navigate

  • Home
  • Current issue
  • Archive

About the ERJ

  • Journal information
  • Editorial board
  • Press
  • Permissions and reprints
  • Advertising

The European Respiratory Society

  • Society home
  • myERS
  • Privacy policy
  • Accessibility

ERS publications

  • European Respiratory Journal
  • ERJ Open Research
  • European Respiratory Review
  • Breathe
  • ERS books online
  • ERS Bookshop

Help

  • Feedback

For authors

  • Instructions for authors
  • Publication ethics and malpractice
  • Submit a manuscript

For readers

  • Alerts
  • Subjects
  • Podcasts
  • RSS

Subscriptions

  • Accessing the ERS publications

Contact us

European Respiratory Society
442 Glossop Road
Sheffield S10 2PX
United Kingdom
Tel: +44 114 2672860
Email: journals@ersnet.org

ISSN

Print ISSN:  0903-1936
Online ISSN: 1399-3003

Copyright © 2023 by the European Respiratory Society